高效液相色谱法检测蛋黄卵磷脂中的磷脂酰胆碱

吉林大学学报(工学版) ›› 2012, Vol. 42 ›› Issue (增刊1): 449-453.

高效液相色谱法检测蛋黄卵磷脂中的磷脂酰胆碱

刘静波¹, 常皓¹, 王二雷¹, 宫新统¹, 姜轶群²

1. 吉林大学军需科技学院营养与功能食品研究室, 长春 130062;
2. 吉林大学生命科学学院,长春 130021

收稿日期:2011-11-22 出版日期:2012-09-01 发布日期:2012-09-01
通讯作者: 姜轶群(1982-),女,讲师,博士.研究方向:生物化学与分子生物学.E-mail:jiangyiq@jlu.edu.cn E-mail:jiangyiq@jlu.edu.cn
作者简介:刘静波(1962-),女,教授,博士生导师.研究方向:营养与功能性食品.E-mail:ljb168@sohu.com
基金资助:
吉林省科技厅农业重点基金项目(20050202-3).

Phosphatidylcholine of egg yolk lecithin detection using high performance liquid chromatography(HPLC)

LIU Jing-bo¹, CHANG Hao¹, WANG Er-lei¹, GONG Xin-tong¹, JIANG Yi-qun²

1. Laboratory of Nutrition and Functional Food, College of Quartermaster Technology, Jilin University, Changchun 130062, China;
2. College of Life Science, Jilin University, Changchun 130021, China

Received:2011-11-22 Online:2012-09-01 Published:2012-09-01

摘要/Abstract

摘要： 为建立一种准确、简便的蛋黄卵磷脂中磷脂酰胆碱的检测方法,采用高效液相色谱-紫外检测法对其进行了研究。SHIMADZU LC-2010高效液相色谱仪配备AGILENT ZORBAX RX-SIL型硅胶柱,在200 nm下对磷脂酰胆碱进行检测,流动相采用简单的甲醇/乙酸二元体系,加入的乙酸溶液改善了磷脂酰胆碱的峰形,有效抑制了严重的拖尾现象。流速较低,为0.5 mL/min,减少了流动相的使用量。采用外标法定量,标准曲线线性范围较宽,为10 ～160 μg/mL。检出限为0.5904 μg/mL,定量限为1.9680 μg/mL。经方法学验证性试验,证明本方法精密度、稳定性良好,准确度较高。

关键词: 食品加工技术, 高效液相色谱, 磷脂酰胆碱, 卵磷脂

Abstract: In order to establish an accurate, simple detection method of phosphatidylcholine of egg yolk lecithin, this research paid attention to high performance liquid chromatography(HPLC)-ultraviolet (UV) detector method. SHIMADZU LC-2010 HPLC equipped with AGILENT ZORBAX RX-SIL silica gel column was used. Detection wave was 200 nm. Simpler binary system of methanol and ethanoic acid was used as mobile phase, and added ethanoic acid improved phosphatidylcholine chromatograph peak shape, tail formation was inhibited efficiently. Flow rate was 0.5 mL/min. It was relatively small and reduced mobile phase usage. External standardization quantization was used, and the standard curve linear range was wide and it was 10 ～160 μg/mL. Detection limit was 0.5904 μg/mL, and quantitative limit was 1.9680 μg/mL. The validation test proved the proposed method is characterized by high precision and good stability.

Key words: food processing technology, high performance liquid chromatography (HPLC), phosphatidylcholine, lecithin

中图分类号:

TS253.1

刘静波, 常皓, 王二雷, 宫新统, 姜轶群. 高效液相色谱法检测蛋黄卵磷脂中的磷脂酰胆碱[J]. 吉林大学学报(工学版), 2012, 42(增刊1): 449-453.

LIU Jing-bo, CHANG Hao, WANG Er-lei, GONG Xin-tong, JIANG Yi-qun. Phosphatidylcholine of egg yolk lecithin detection using high performance liquid chromatography(HPLC)[J]. 吉林大学学报(工学版), 2012, 42(增刊1): 449-453.

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