细丝蛋白A对药物诱导的乳腺癌细胞凋亡的影响

J4 ›› 2011, Vol. 37 ›› Issue (6): 1070-1074.

细丝蛋白A对药物诱导的乳腺癌细胞凋亡的影响

李小翠^1, 袁博^*, 刘秀花¹, 赵玲玲¹,王树东², 袁长吉¹

1.吉林大学第一医院肿瘤中心,吉林长春130021；2.吉林大学第一医院心血管中心|吉林长春 130021

收稿日期:2011-09-05 出版日期:2011-11-28 发布日期:2011-11-28
通讯作者: 袁长吉（Tel：0431-88782811，E-mail：yuanchangji@163.com) E-mail:yuanchangji@163.com
作者简介:李小翠(1986-)|女|辽宁省铁岭市人|在读医学硕士|主要从事肿瘤的诊断及治疗研究。
基金资助:
吉林省科技厅自然科学基金资助课题（201115048）

Influence of filamin A on |drug-induced apoptosis of breast cancer cells

LI Xiao-cui¹|YUAN Bo*|LIU Xiu-hua¹|ZHAO Ling-ling¹|WANG Shu-dong²,YUAN Chang-ji¹

1.Tumor Center,First Hospital,Jilin University,Changchun 130021,China；2.Department of Cardiovascular Medicine,First Hospital,Jilin University,Changchun 130021,China

Received:2011-09-05 Online:2011-11-28 Published:2011-11-28

摘要/Abstract

摘要：

目的: 研究细丝蛋白A(FLNa)对药物诱导的乳腺癌细胞凋亡的影响,探讨其影响细胞凋亡的具体机制。方法: 不同浓度(2.5、5.0、10.0和20.0 μmol?L-1)的氯化甲基汞(MMC)、三氧化二砷(AS2O3)及顺铂(DDP)分别作用MDA-MB-231/MB 231-KD(FLNa+/FLNa－)2种乳腺癌细胞，同时设空白对照组，采用MTT比色法检测各组乳腺癌细胞增殖抑制率。不同浓度(2.5、5.0、10.0和20.0 μmol?L-1)MMC分别作用MDA-MB-231/MB 231-KD(FLNa+/ FLNa－)2种乳腺癌细胞，同时设空白对照组，用流式细胞仪测定各组乳腺癌细胞的细胞凋亡率。结果：与空白对照组比较，2.5、5.0、10.0及20.0 μmol?L-1MMC组FLNa+和FLNa－细胞的增殖抑制率明显增加(P<0.05)，与AS2O3及DDP组比较抑制率也明显增加(P<0.05)；2种乳腺癌细胞之间比较，FLNa－乳腺癌细胞较FLNa+细胞抑制率明显增高 (P<0.05)。流式细胞术结果显示，随着MMC剂量的增加凋亡细胞数增加，与空白对照组比较差异均有统计学意义(P<0.05)，FLNa－细胞较FLNa+细胞凋亡率高,差异有统计学意义(P<0.05)。结论： FLNa的表达可以使药物诱导的乳腺癌细胞凋亡作用减弱。

关键词: 细丝蛋白A；细胞凋亡；乳腺肿瘤

Abstract:

Objective
To investigate the influence of filamin A(FLNa) on drug-i
nduced apoptosis of breast cancer cells and explore its mechanism.Methods MDA-MB-231/MB 231-KD(FLNa+/ FLNa－) cells were treated with different doses(2.5,5.0,10.0 and 20.0 μmol?L-1)MMC,AS2O3 and DDP,meanwhile control group was set up.The inhibitory rates of proliferation of breast cancer cells were detected with MTT method.MDA-MB-231/MB231-KD(FLNa+/FLNa－)cells were treated with different doses (2.5，5.0，10.0 and 20.0 μmol?L-1)MMC,meanwhile control group was set up.The apoptotic rates of breast cancer cells were determined with flow cytometry.Results Compared with control group,the inhibitory rates of proliferation of FLNa+ and FLNa－ cells in 2.5,5.0,10.0,20.0 μmol?L-1 MMC groups were significantly increased(P<0.05),and were higher than those in AS2O3 and DDP groups(P<0.05)；there was significant difference between two kinds of breast cancer cells，the inhibitory rate of FLNa－ cells was higher than that of FLNa+ cells (P<0.05).As the dose increasing, the number of apoptotic cells was increased,there were significant differences compared with control group(P<0.05);the inhibitory rate of FLNa－ cells was higher than that of FLNa＋ cells and there was significant difference (P<0.05).Conclusion The expression of FLNa can reduce the drug-induced apoptosis of breast cancer cells.

Key words: filamin A； apoptosis；breast neoplasms

中图分类号:

R737.9

李小翠, 袁博, 刘秀花, 赵玲玲,王树东, 袁长吉. 细丝蛋白A对药物诱导的乳腺癌细胞凋亡的影响[J]. J4, 2011, 37(6): 1070-1074.

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