关键词: 层黏连蛋白, 不育症, 女(雌)性, 免疫组织化学

Abstract: Objective To investigate the defect in embedding of female inferitility, especially the abnormity of the endometrium acceptance. Methods Immunohistochemical method was used to test the expressions of extra cellar matrix proteins-collagen type Ⅳ (COLⅣ) and laminin (LN) on the secretory phase endometrium of 41 fertile women (fertility group)and 43 unexplained infertile women (infertility group). Results The expressions of COLⅣ and LN in fertility group were strong in early secretory phase, while their expressions were the strongest in mid-term secretory phase and the weakest in late secretory phase. The expressions of COLⅣ and LN in every secretory phase in the infertility group were stronger than that in the fertility group, the expressions of COLⅣ and LN in the secertory phase endometrium in the fertility group was different from that in the infertility group. The expressions of COLⅣ and LN in gland-epithelia of the endometrium and the basemembrance, the blood vessel endothelial basemembrance and the interstitial cells in the same group were different. Conclusion The COLⅣ and LN expressions in the secertory phase change periodically and participate in the function change of the endometrium and the embedding of the blastocyst. Their stronger expressions affect the identification,location, and adherence to the endometrium of marix,and lead to the failure of the blastocyst embedding.

Key words: laminin, infertility, female, immunohistochemistry