TGF-β1和PLGF在不同类型妊娠滋养细胞疾病患者病变组织中的表达及其意义

doi:10.13481/j.1671-587x.20180528

吉林大学学报(医学版) ›› 2018, Vol. 44 ›› Issue (05): 1041-1046.doi: 10.13481/j.1671-587x.20180528

TGF-β1和PLGF在不同类型妊娠滋养细胞疾病患者病变组织中的表达及其意义

李红霞¹, 宫海叶¹, 曾萧¹, 张素素², 刘伯锋³

1. 延安大学附属医院妇科, 陕西延安 716000;
2. 江西省新余市妇幼保健院妇科, 江西新余 338025;
3. 延安大学医学院解剖教研室, 陕西延安 716000

收稿日期:2017-11-09 出版日期:2018-09-28 发布日期:2018-11-20
通讯作者: 刘伯锋,副教授(Tel:0911-2881423,E-mail:lihx66@163.com) E-mail:lihx66@163.com
作者简介:李红霞(1972-),女,陕西省延安市人,副教授,医学硕士,主要从事妇科肿瘤临床方面的研究。
基金资助:
陕西省教育厅科研项目资助课题（2013JK0782）；陕西省延安市科技惠民计划项目资助课题（2017HM-02-02）；延安大学研究生教学改革研究项目资助课题（YDYJG2017014）

Expressions of TGF-β1 and PLGF in tissue of patients with different types of gestational trophoblastic diseases and their significances

LI Hongxia¹, GONG Haiye¹, ZENG Xiao¹, ZHANG Susu², LIU Bofeng³

1. Department of Gynecology, Affiliated Hospital, Yan'an University, Yan'an 716000, China;
2. Department of Gynecology, Xinyu Maternal and Child Health Hospital, Jangxi Province, Xinyu 338025, China;
3. Department of Anatomy, College of Medical Sciences, Yan'an University, Yan'an 716000, China

Received:2017-11-09 Online:2018-09-28 Published:2018-11-20

摘要/Abstract

摘要： 目的：检测不同类型妊娠滋养细胞疾病患者病变组织中转化生长因子β1（TGF-β1）和胎盘生长因子（PLGF）的表达，阐明其在不同妊娠滋养细胞疾病中意义。方法：收集葡萄胎组织40例（葡萄胎组）、妊娠滋养细胞肿瘤组织26例（GTN组，包括侵蚀性葡萄胎22例和绒毛膜癌4例）和正常早孕绒毛组织40例（正常对照组）作为研究对象。采用免疫组织化学法检测不同组织中TGF-β1和PLGF的阳性表达率，分析TGF-β1和PLGF在正常对照组、葡萄胎组和GTN组患者中的表达差异以及其与患者临床高危因素（年龄>40岁、子宫体积>孕周和卵巢黄素化囊肿直径>6 cm）的关联性。结果：与正常对照组（87.5%）比较，葡萄胎组（62.5%）和GTN组（30.8%）TGF-β1阳性表达率明显降低（P<0.05）；且葡萄胎组TGF-β1阳性表达率高于GTN组（P<0.05）。与正常对照组（22.5%）比较，葡萄胎组（50.0%）和GTN组（80.8%）PLGF阳性表达率明显升高（P<0.05），且葡萄胎组PLGF阳性表达率低于GTN组（P<0.05）。与正常对照组比较，葡萄胎组患者中年龄>40岁、子宫体积>孕周、卵巢黄素化囊肿直径>6 cm者TGF-β1阳性表达率明显降低（P<0.05），而PLGF阳性表达率明显升高（P<0.05）。在GTN组中并发高危因素者较无高危因素者TGF-β1阳性表达率明显降低（P<0.05），而PLGF阳性表达率明显升高（P<0.05）。葡萄胎组和GTN组患者TGF-β1与PLGF阳性表达水平均呈负相关关系（r=-0.585，P<0.05；r=-0.479，P<0.05）。结论：TGF-β1在葡萄胎和妊娠滋养细胞肿瘤组织中阳性表达逐渐降低，PLGF表达率逐渐升高，可能与妊娠滋养细胞疾病患者的不同类型及预后有关。

关键词: 妊娠滋养细胞疾病, 转化生长因子β1, 胎盘生长因子, 免疫组织化学

Abstract: Objective:To detect the expressions of transforming growth factor-β1 (TGF-β1) and placental growth factor (PLGF) expressions in the tissue of the patients with different types of gestational trophoblastic diseases,and to clarfy the significances of their expressions in different types of gestational trophoblastic diseases. Methods:A total of 40 cases of hydatidiform mole tissue (hydatidiform mole group) and 26 cases of gestational trophoblastic tumour tissue (GTN group, including 22 cases of invasive hydatidiform mole and 4 cases of choriocarcinoma), and 40 cases of normal early pregnancy villi (normal control group) were collected.Immunohistochemical method was used to detect the positive expression rates of TGF-β1 and PLGF, the differences of TGF-β1 and PLGF expressions between normal group, hydatidiform mole group and GTN group were analyzed, and the associations between them and the clinical high risk factors(age>40 years old, the uterine volume> gestational age, and thecalutein ovarian cyst diameter>6 cm) were analyzed. Results:The positive expression rates of TGF-β1 in hydatidiform mole group(62.5%)and GTN group(30.8%) were lower than that in normal group(87.5%) (P<0.05);the positive expression rates of TGF-β1 in hydatidiform mole group was higher than that in GTN group(P<0.05).The positive expression rates of PLGF in hydatidiform mole group(50.5%) and GTN group(80.8%%) were higher than that in normal control group(22.5%%);the positive expression rate of PLGF in hydatidiform mole group was lower than that in GTN group (P<0.05).The positive expression rates of TGF-β1 of the patients in hydatidiform mole group with the age>40 years old, the uterine volume>the gestational age,and the ovarian luteinized cyst diameter>6 cm were significantly decreased (P<0.05) and the positive expression rates of PLGF were significantly increased (P<0.05) compared with normal control group.The positive expression rate of TGF-β1 of the patients in GTN group with the high risk factors was significantly decreased compared with those without the high risk factors(P<0.05) and the positive expression rate of PLGF was significantly increased (P<0.05).In hydatidiform mole group and GTN group, there were moderately negative correlations between the positive expression rates of TGF-β1 and PLGF (r=-0.585,P<0.05;r=-0.479, P<0.05). Conclusion:The expression levels of TGF-β1 in hydatidiform mole and gestational trophoblastic tumor are gradually decreased, and the expression levels of PLGF are gradually increased, which may be related to the different types and prognosis of gestational trophoblastic diseases.

Key words: gestational trophoblastic disease, transforming growth factor-β1, placental growth factor, immunohistochemistry

中图分类号:

R737.33

李红霞, 宫海叶, 曾萧, 张素素, 刘伯锋. TGF-β1和PLGF在不同类型妊娠滋养细胞疾病患者病变组织中的表达及其意义[J]. 吉林大学学报(医学版), 2018, 44(05): 1041-1046.

LI Hongxia, GONG Haiye, ZENG Xiao, ZHANG Susu, LIU Bofeng. Expressions of TGF-β1 and PLGF in tissue of patients with different types of gestational trophoblastic diseases and their significances[J]. Journal of Jilin University(Medicine Edition), 2018, 44(05): 1041-1046.

参考文献

[1] 向阳, 赵峻.妊娠滋养细胞疾病诊治进展[J]. 中国实用妇科与产科杂志, 2017, 33(1):14-18.
[2] Braga A, Maesta L, Short D, et al. Hormonal contraceptive ust before hcg remission does not increase the risk of gestational trophoblastic neoplasia following complete hydatidiform mole:a historical database review[J]. BJOG, 2016,123(8):1330-1335.
[3] Lu B, Zhang X, Liang Y. Clinicopathologic analysis of postchemotherapy gestational trophoblastic neoplasia:an entity overlapping with epithelioid trophoblastic tumor[J]. Int J Gynecol Pathol,2016,35(6):516-524.
[4] Brooks SA, Martin E, Smeester L, et al. miRNAs as common regulators of the transforming growth factor (TGF)-β pathway in the preeclamptic placenta and cadmium-treated trophoblasts:Links between the environment, the epigenome and preeclampsia[J]. Food Chem Toxicol, 2016, 98(Pt A):50-57.
[5] Bolat F, Haberal N, Tunali N, et al. Expression of vascular endothelial growth factor (VEGF), hypoxia inducible factor 1 alpha (HIF-1a), and transforming growth factors β1(TGFβ1) and β3(TGFβ3) in gestational trophoblastic disease[J]. Pathol Res Prac, 2010, 206(1):19-23.
[6] Singh MK, Bhattacharya D, Chaudhuri S, et al. T11TS inhibits glioma angiogenesis by modulation of MMPs, TIMPs, with related integrin αv and TGF-β1 expressions[J]. Tumour Biol, 2014, 35(3):2231-2246.
[7] 刘绍晨, 许倩, 岳志领.TGF-β1对人绒癌JEG-3细胞株TβRⅠ、TβRⅡ、Smad4 mRNA表达的影响及意义[J]. 中国妇幼保健, 2015, 30(5):758-763.
[8] Fu G, Ye G, Nadeem L, et al. MicroRNA-376c impairs transforming growth factor-β and nodal signaling to promote trophoblast cell proliferation and invasion[J]. Hypertension, 2013, 61(4):864-872.
[9] Singh MK, Bhattacharya D, Chaudhuri S, et al. T11TS inhibits glioma angiogenesis by modulation of MMPs, TIMPs, with related integrin αv and TGF-β1 expressions[J]. Tumour Biol, 2014, 35(3):2231-2246.
[10] Wu Y, Fu Y, Zheng L, et al. Nutlin-3 inhibits epithelial-mesenchymal transition by interfering with canonical transforming growth factor-β1-Smad-Snail/Slug axis[J]. Cancer Lett, 2014, 342(1):82-91.
[11] Wu XD, Chen LL,Shen T, et al. Curative effects and influenced factors of primary chemotherapy with single-agent methotrexate on low-risk gestational trophoblastic neoplasia[J].Zhonghua Yi Xue Za Zhi, 2017, 97(23):1769-1772.
[12] Schneider K, Weyerbrock A, Doostkam S, et al. Lack of evidence for PLGF mediating the tumor resistance after anti-angiogenic therapy in malignant gliomas[J]. J Neuro Oncol, 2015, 121(2):269-278.
[13] Vuorela P, Hatva E, Lymboussaki A, et al. Expression of vascular endothelial growth factor and placenta growth factor in human placenta[J].Biol Reprod,1997,56(2):489-494.
[14] Xiang L,Varshney R,Rashdan NA, et al. Placental growth factor and vascular endothelial growth factor-A have differential, cell-type specific patterns of expression in vascular cells[J]. Microcirculation,2014, 21(5):368-379.
[15] Tsiakkas A, Duvdevani N, Wright A, et al. Serum placental growth factor in the three trimesters of pregnancy:effects of maternal characteristics and medical history[J]. Ultrasound Obstet Gynecol, 2015, 45(5):591-598.
[16] Song N, Liu H, Ma X, et al. Placental growth factor promotes metastases of ovarian cancer through MiR-543-regulated MMP7[J]. Cell Physiol Biochem, 2015, 37(3):1104-1112.
[17] 李润花, 郑兆祥, 武明霞, 等. 胎盘生长因子及瘦素在早孕绒毛及妊娠滋养细胞疾病中的表达及意义[J]. 中华妇幼临床医学杂志:电子版, 2012, 8(4):485-488.
[18] Oranratanaphan S, Wongwathanavikrom R, Lertkhachonsuk R. Correlation levels of serum placental growth factor and human chorionic gonadotropin in gestational trophoblastic disease patients[J]. J Reprod Med, 2011, 56(7/8):333-338.
[19] De Falco S. The discovery of placenta growth factor and its biological activity[J]. Exp Mol Med, 2012, 44(1):1-9.
[20] Singh M, Kindelberger D, Nagymanyoki Z, et al. Vascular endothelial growth factors and their receptors and regulators in gestational trophoblastic diseases and normal placenta[J]. J Reprod Med, 2012, 57(5/6):197-203.
[21] Incio J, Tam J, Rahbari NN, et al. PLGF/VEGFR-1 signaling promotes macrophage polarization and accelerated tumor progression in obesity[J]. Clin Cancer Res, 2016, 22(12):2993-3004.
[22] Shamshiri Milani H, Abdollahi M, Torbati S, et al. Risk factors for hydatidiform mole:Is husband's job a major risk factor[J].Asian Pac J Cancer Prev,2017, 18(10):2657-2662.
[23] Giorgione V, Bergamini A, Cioffi R, et al.Role of surgery in the management of hydatidiform mole in elderly patients:a single-center clinical experience[J]. Int J Gynecol Cancer,2017,27(3):550-553.

TGF-β1和PLGF在不同类型妊娠滋养细胞疾病患者病变组织中的表达及其意义

Expressions of TGF-β1 and PLGF in tissue of patients with different types of gestational trophoblastic diseases and their significances

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	赵振理, 畅继武, 孙光, 胡少华. AAX14401蛋白在人膀胱癌组织中的表达及其意义[J]. 吉林大学学报(医学版), 2018, 44(05): 1052-1055.
[2]	王天月, 崔晓燕, 吴骁, 王丹. 磷酸化胞外信号调节激酶在小鼠海马发育过程中的表达及其意义[J]. 吉林大学学报(医学版), 2018, 44(05): 979-982.
[3]	张啸环, 段明华, 闫玉礼, 潘新, 李海清, 周长龙, 赵天倚. 人羊膜成纤维细胞的分离和鉴定[J]. 吉林大学学报(医学版), 2018, 44(04): 845-848.
[4]	阎慧, 娄石磊, 苗永迪, 岂蕊, 郭子硕, 邱悦, 孙聪. 大鼠结肠原代成纤维细胞的分离、培养和鉴定[J]. 吉林大学学报(医学版), 2018, 44(04): 849-852.
[5]	包幸福, 倪宇昕, 李莹, 郭小凯, 王玉琢, 方蛟, 胡敏. Wnt5a在小鼠牙骨质发育中的表达特点及其对成牙骨质细胞分化的影响[J]. 吉林大学学报(医学版), 2018, 44(03): 461-465.
[6]	马晓周, 刘麒麟, 尹唯凰, 王越, 于阳, 杨刚, 于子莹. CC趋化因子受体6在人唾液腺腺样囊性癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2018, 44(01): 147-150.
[7]	曹艳莎, 李婳, 陈明红, 刘宝琴, 王华芹, 李宁. 组织芯片技术检测BAG3蛋白在结肠癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2017, 43(06): 1177-1181.
[8]	高新雅, 张伟杰, 崔黎, 周学良, 马志俊, 吕转, 池艳艳, 王留兴. TM4SF1在乳腺癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2017, 43(06): 1186-1192.
[9]	周世平, 徐红, 王琇, 李蓥. 内镜黏膜下剥离术治疗食管颗粒细胞瘤2例报告并文献复习[J]. 吉林大学学报(医学版), 2017, 43(05): 1034-1036.
[10]	历春, 付天悦, 杨宝学, 盖晓东. 尿素通道蛋白B在人膀胱癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2017, 43(04): 705-708.
[11]	石俊忠, 庄建彬, 张传山, 刘贤明, 孔大陆. β-tubulin Ⅲ在结肠腺癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2017, 43(04): 770-775.
[12]	文爱平, 汪海娇, 金景姬, 蔡勇, 朱继红, 谌伦华. 子宫内膜癌组织中hMOF蛋白和mRNA表达及其临床意义[J]. 吉林大学学报(医学版), 2016, 42(06): 1138-1142.
[13]	温泉, 王园园, 司鹤南, 田亚萍. 成人郎格汉斯细胞组织细胞增生症1例报告及文献复习[J]. 吉林大学学报(医学版), 2016, 42(05): 985-987.
[14]	郭莉, 于美玲, 韩潇, 张晓霞. 卵巢非特异性类固醇细胞肿瘤1例报告及文献复习[J]. 吉林大学学报(医学版), 2016, 42(05): 988-990.
[15]	孔桂美, 王继军, 陶文华, 卜平, 钱锋. 组蛋白精氨酸甲基转移酶5在胃癌组织中的表达及其意义[J]. 吉林大学学报(医学版), 2016, 42(04): 753-756.