AAX14401蛋白在人膀胱癌组织中的表达及其意义

doi:10.13481/j.1671-587x.20180530

吉林大学学报(医学版) ›› 2018, Vol. 44 ›› Issue (05): 1052-1055.doi: 10.13481/j.1671-587x.20180530

AAX14401蛋白在人膀胱癌组织中的表达及其意义

赵振理¹, 畅继武², 孙光², 胡少华¹

1. 海南省妇幼保健院泌尿外科, 海南海口 571199;
2. 天津市泌尿外科研究所肿瘤免疫研究所, 天津 300202

收稿日期:2017-12-06 出版日期:2018-09-28 发布日期:2018-11-20
通讯作者: 畅继武,教授,博士研究生导师(Tel:020-28331756,E-mail:jwchang_cirl@163.com);赵振理,副主任医师,硕士研究生导师(Tel:0898-36615520,E-mail:zzl8287@163.com) E-mail:jwchang_cirl@163.com;zzl8287@163.com
作者简介:赵振理(1970-),男,山西省太原市人,副主任医师,医学博士,主要从事泌尿系肿瘤方面的研究。
基金资助:
国家自然科学基金资助课题（30371601）

Expression of AAX14401 in human bladder cancer tissue and its significance

ZHAO Zhenli¹, CHANG Jiwu², SUN Guang², HU Shaohua¹

1. Department of Urinary Surgery, Maternity and Child Health Care Hospital of Hainan Province, Haikou 571199, China;
2. Department of Tumor Immunity, Tianjin Institute of Urinary Surgery, Tianjin 300202, China

Received:2017-12-06 Online:2018-09-28 Published:2018-11-20

摘要/Abstract

摘要： 目的：研究AAX14401蛋白在正常膀胱组织和膀胱移行细胞癌组织中的表达情况，分析其在膀胱癌发生发展过程中的作用。方法：收集膀胱癌组织标本75例作为实验组，以正常膀胱黏膜组织12例作为对照组，应用免疫组织化学法检测谷胱甘肽S转移酶P1（GSTP1）和AAX14401的阳性表达率，分析GSTP1和AAX14401蛋白阳性表达率在膀胱癌组织和正常膀胱组织中的差异。结果：正常膀胱组织中GSTP1蛋白阳性表达率为58.3%，膀胱癌组织中GSTP1蛋白阳性表达率为90.7%，二者比较差异有统计学意义（P<0.01）；在正常膀胱组织中AAX14401蛋白表达均为阴性，在膀胱癌组织中AAX14401蛋白阳性表达率为2.7%，二者比较差异无统计学意义（P=0.596）；膀胱癌组织中AAX14401蛋白和GSTP1蛋白无同为阳性表达者，GSTP1阴性表达的膀胱癌组织中有2例Anti-AY阳性表达，GSTP1蛋白与AAX14401蛋白在膀胱癌组织中阳性表达呈负相关关系（r=-0.274，P=0.018）。结论：AAX14401蛋白在膀胱癌中的表达可能促进了膀胱癌的发生发展。

关键词: 膀胱肿瘤, AAX14401蛋白, 谷胱甘肽S转移酶P1, 免疫组织化学, Anti-AY抗体

Abstract: Objective:To study the expressions of AAX14401 in the normal bladder tissueand the human bladder transitional cell carcinoma tissue, and to analyze its function in the occurrence and development of bladder cancer. Methods:Seventy-five cases of specimens of bladder transitional cell carcinoma were collected and used as experimental group and 12 cases of normal bladder mucosa specimens were selected as control group. The positive expression rates of glutathione S-transferase pi-1(GSTP1) and AAX14401 were detected by immunohistochemistry. The differences of positive expression rates of GSTP1 and AAX14401 proteins between bladder cancer tissue and normal bladder tissue were analyzed. Results:The positive expression rate of GSTP1 protein in normal bladder tissue was 58.3%, while it was 90.7% in bladder cancer tissue, and the difference between them was significant (P<0.01). The positive expression rate of AAX14401 protein in normal bladder tissue was 0%, while it was 2.7% in blandder cancer tissue, and there was no significant difference between them(P=0.596). There was no one with positive AAX14401 expression and positive GSTP1 expression in bladder cancer tissue at the same time. There were 2 cases with positive AAX14401 expression in bladder cancer tissue with negative GSTP1 expression; there was a negative correlation between the positive expressions of AAX14401 and GSTP1 proteins in bladder cancer (r=-0.274, P=0.018). Conclusion:The expression of AAX14401 protein may promote the incidence and progression of bladder cancer.

Key words: bladder neoplasms, AAX14401 protein, glutathione S-transferase P-1, immunohistochemistry, Anti-AY antibody

中图分类号:

R737.14

赵振理, 畅继武, 孙光, 胡少华. AAX14401蛋白在人膀胱癌组织中的表达及其意义[J]. 吉林大学学报(医学版), 2018, 44(05): 1052-1055.

ZHAO Zhenli, CHANG Jiwu, SUN Guang, HU Shaohua. Expression of AAX14401 in human bladder cancer tissue and its significance[J]. Journal of Jilin University(Medicine Edition), 2018, 44(05): 1052-1055.

参考文献

[1] 赵振理,畅继武,孙光.AY887902全长扩增及其生物信息学研究[J].中国药物与临床,2014,14(1):5-8.
[2] 胡少华,赵振理,孙光,等.蛋白AAX14401相应抗体的制备及鉴定[J].临床医药实践,2015,24(9):683-685.
[3] Ferlay J, Soerjomataram I, Dikshit R, et al. Cancer incidence and mortality worldwide:sources, methods and major patterns in GLOBOCAN 2012[J].Int J Cancer, 2015, 136(5):E359-386.
[4] Yang SJ, Wang DD, Li J, et al. Predictive role of GSTP1-containing exosomes in chemotherapy-resistant breast cancer[J]. Gene, 2017, 623:5-14.
[5] Sawers L, Ferguson MJ, Ihrig BR, et al. Glutathione S-transferase P1(GSTP1) directly influences platinum drug chemosensitivity in ovarian tumour cell lines[J]. Br J Cancer, 2014, 111(6):1150-1158.
[6] Fang C, Wei XM, Zeng XT, et al. Aberrant GSTP1 promoter methylation is associated with increased risk and advanced stage of breast cancer:a meta-analysis of 19 case-control studies[J]. BMC Cancer, 2015, 15:920.
[7] Gu JD, Hua F, Mei CR, et al. HapMap-based study on the association between MPO and GSTP1 gene polymorphisms and lung cancer susceptibility in Chinese Han population[J]. Acta Pharmacol Sin, 2014, 35(5):636-644.
[8] Kumamoto K, Ishibashi K, Okada N, et al. Polymorphisms of GSTP1, ERCC2 and TS-3'UTR are associated with the clinical outcome of mFOLFOX6 in colorectal cancer patients[J]. Oncol Lett, 2013, 6(3):648-654.
[9] Zhang J, Grek C, Ye ZW, et al. Pleiotropic functions of glutathione S-transferase P[J]. Adv Cancer Res, 2014, 122:143-175.
[10] Djukic T, Simic T, Pljesa-Ercegovac M, et al. Upregulated glutathione transferase omega-1 correlates with progression of urinary bladder carcinoma[J]. Redox Rep, 2017, 22(6):486-492.
[11] 唐荣金,何小龙,朱海琴,等.GSTP1表达下调促进膀胱癌细胞T24凋亡[J].安徽医科大学学报,2017,52(10):1463-1467.
[12] Uchida Y, Chiyomaru T, Enokida H, et al. MiR-133a induces apoptosis through direct regulation of GSTP1 in bladder cancer cell lines[J]. Urol Oncol, 2013, 31(1):115-123.
[13] Wang Z, Xue L, Chong T, et al. Quantitative assessment of the association between glutathione S-transferase P1 Ile105Val polymorphism and bladder cancer risk[J]. Tumour Biol, 2013, 34(3):1651-1657.
[14] Pljesa-Ercegovac M, Savic-Radojevic A, Dragicevic D, et al. Enhanced GSTP1 expression in transitional cell carcinoma of urinary bladder is associated with altered apoptotic pathways[J]. Urol Oncol, 2011, 29(1):70-77.
[15] Wang CS, Wei SJ, Zhao GG. Correlation of sensitivity of chemotherapy drugs with the originated tissue, clinical stage and pathological type of carcinoma[J]. Sect Clin Biochem Lab Med Foreign Med Sci, 2005, 26(4):209-212.
[16] 夏冰,陈晓东,刘大志,等.乳腺癌化疗药物的体外敏感性与临床病理的关系[J].肿瘤研究与临床,2008,20(11):745-748.
[17] Gao L, Fang YQ, Zhang TY, et al.GSTP1 arrests bladder cancer T24 cells in G0/G1 phase and up-regulates p21 expression[J]. Int J Clin Exp Med, 2014, 7(9):2984-2991.
[18] Berendsen CL, Peters WH, Scheffer PG, et al. Glutathione S-transferase activity and subunit composition in transitional cell cancer and mucosa of the human bladder[J].Urology,1997,49(4):644-651.

AAX14401蛋白在人膀胱癌组织中的表达及其意义

Expression of AAX14401 in human bladder cancer tissue and its significance

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