Matrilin-4对大鼠牙髓损伤后修复性牙本质形成的促进作用

doi:10.13481/j.1671-587x.20200113

吉林大学学报(医学版) ›› 2020, Vol. 46 ›› Issue (01): 73-77.doi: 10.13481/j.1671-587x.20200113

Matrilin-4对大鼠牙髓损伤后修复性牙本质形成的促进作用

谢金芳¹, 孟琳², 高华丽³, 李雪洋³, 胡雪³, 张颖丽³, 尹硕⁴

1. 吉林大学口腔医院综合口腔治疗科, 吉林长春 130021;
2. 吉林大学口腔医院病理科, 吉林长春 130021;
3. 吉林大学口腔医院牙体牙髓科, 吉林长春 130021;
4. 吉林省长春市口腔医院修复科, 吉林长春 130022

收稿日期:2019-04-12 出版日期:2020-01-28 发布日期:2020-02-03
通讯作者: 张颖丽,教授,硕士研究生导师(Tel:0431-88796017,E-mail:zhangyingli1989@163.com);尹硕,主治医师(Tel:0431-88742473,E-mail:934232260@qq.com) E-mail:zhangyingli1989@163.com;934232260@qq.com
作者简介:谢金芳(1992-),女,黑龙江省延寿县人,医师,医学硕士,主要从事牙体牙髓疾病基础和临床方面的研究。
基金资助:
吉林省卫健委科研基金资助课题（DBXM111-2017）；吉林省教育厅科研项目资助课题（JJKH20180229KJ）

Promotion effect of Matrilin-4 on reparative dentin formation after dental pulp injury in rats

XIE Jinfang¹, MENG Lin², GAO Huali³, LI Xueyang³, HU Xue³, ZHANG Yingli³, YIN Shuo⁴

1. Department of General Dentistry, Stomatology Hospital, Jilin University, Changchun 130021, China;
2. Department of Oral Pathology, Stomatology Hospital, Jilin University, Changchun 130021, China;
3. Department of Endodontics, Stomatology Hospital, Jilin University, Changchun 130021, China;
4. Changchun Stomatology Hospital, Jilin Province, Changchun 130022, China

Received:2019-04-12 Online:2020-01-28 Published:2020-02-03

摘要/Abstract

摘要： 目的：研究Matrilin-4对大鼠牙髓损伤后修复性牙本质形成的作用，探讨其作为新型盖髓剂的可能性。方法：28只雄性Wistar大鼠，在大鼠的双侧上颌第一磨牙牙合面备洞至露髓后，左侧用含Matrilin-4的胶原蛋白海绵盖髓（Matrilin-4组），右侧用含磷酸盐缓冲溶液（PBS）的胶原蛋白海绵盖髓（PBS组），双侧均用玻璃离子封洞。分别于3、7、14和28 d灌流固定处死大鼠并取上颌双侧第一磨牙标本，HE染色和免疫组织化学染色观察分析各组大鼠修复性牙本质形成及成牙本质细胞中牙本质涎蛋白（DSP）的表达情况。结果：HE染色，3 d时与PBS组比较，Matrilin-4组穿髓孔下方较少量炎症细胞聚集，并可见明显的血管扩张；7 d时2组穿髓孔下方炎症反应加重，但Matrilin-4组明显轻于PBS组，且可见前期牙本质形成；14 d时Matrilin-4组露髓区域可被较连续完整的修复性牙本质桥覆盖；28d时，与PBS组比较，Matrilin-4组穿髓孔下方可见厚而完整的修复性牙本质桥，牙本质桥下面有重组的成牙本质细胞层。免疫组织化学染色，2组大鼠成牙本质细胞中DSP阳性表达强度在术后3、7和14 d呈逐渐增强趋势，28 d时减弱；盖髓后各时间点Matrilin-4组大鼠成牙本质细胞中DSP阳性表达强度均强于PBS组，且14 d时阳性表达达高峰。与PBS组比较，3、7和14 d时Matrilin-4组大鼠成牙本质细胞中DSP阳性表达强度明显升高（P<0.01）。结论：Matrilin-4对牙髓损伤后修复性牙本质的形成具有促进作用。

关键词: Matrilin-4, 牙髓损伤修复, 盖髓剂, 成牙本质细胞

Abstract: Objective: To study the effect of Matrilin-4 on the reparative dentin formation in the rats after dental pulp injury, and to explore its possibility to be used as a new capping agent. Methods: A total of 28 male Wistar rats were selected. A cavity on the occlusal surface of maxillary first molars on both sides was prepared in each rat. Matrilin-4 was applied on the left exposed pulp(Matrilin-4 group), and PBS was applied on the right exposed pulp(PBS group);and the cavities in both sides were covered with glass ions. On days 3, 7, 14 and 28 after operation, the rats were sacrificed,and the maxillary first molars on both sides of the rats were selected. HE staining and immunohistochemistry staining were used to observe and analyze the reparative dentin formation and the expression of dentin sialoprotein(DSP) in the odontoblasts. Results: The HE staining results showed that there were less inflammatory cells under the exposed pulp in that Matrilin-4 group at 3 d after operation compared with PBS group, and obvious vasodilation was found. At 7 d after operation, the inflammatory reaction under the exposed pulps in two groups was aggravated, but the inflammatory reaction in Matrilin-4 group was significantly lighter than that in PBS group, and the prophase dentin formation was found. After injury of 14 d,the pulp-exposed areas were covered by more complete dentin bridge in Matrilin-4 group; at 28 d after operation, compared with PBS group, there was a thick, complete and reparative dentin bridge under the exposed pulps, composed of tubular dentin in Matrilin-4 group, and there were reorganized odontoblastic layers beneath the dentin bridge. The results of immunohistochemistry showed that the positive expression strengths of DSP in the odontoblasts of the rats in two groups were gradually increased at 3, 7 and 14 d after operation, and were decreased at 28 d. The positive expression strengths of DSP in the odontoblast of the rats in Matrilin-4 group were stronger than those in PBS group at each time point after operation, and the positive expression strength reached the peak at 14 d. Conclusion: Compared with PBS group, the positive expression strength of DSP in the odontoblasts of the rats in Matrilin-4 group had significant differences (P<0.01) at 3, 7 and 14 d.

Key words: Matrilin-4, dental pulp injury repair, pulp capping agent, odontoblasts

中图分类号:

R781.3

谢金芳, 孟琳, 高华丽, 李雪洋, 胡雪, 张颖丽, 尹硕. Matrilin-4对大鼠牙髓损伤后修复性牙本质形成的促进作用[J]. 吉林大学学报(医学版), 2020, 46(01): 73-77.

XIE Jinfang, MENG Lin, GAO Huali, LI Xueyang, HU Xue, ZHANG Yingli, YIN Shuo. Promotion effect of Matrilin-4 on reparative dentin formation after dental pulp injury in rats[J]. Journal of Jilin University(Medicine Edition), 2020, 46(01): 73-77.

参考文献

[1] PARISAY I, GHODDUSI J, FORGHANI M. A review on vital pulp therapy in primary teeth[J]. Iran Endod J, 2015, 10(1):6-15.
[2] PARVIN M K, MORAL A A, SHIKDER Z H,et al. Evaluation of radiological outcomes of theracal light cured (TLC) and calcium hydroxide as indirect pulp capping agents in the treatment of deep carious lesion of permanent molar teeth[J]. Mymansingh Med J, 2018, 27(4):859-865.
[3] FRANSSON H. On the repair of the dentine barrier[J]. Swed Dent J Suppl, 2012, 226:9-84.
[4] ZAKARIAMN,CAHYANTOA,EL-GHANNAMA. Calcium release and physical properties of modified carbonate apatite cement as pulp capping agent in dental application[J]. Biomater Res, 2018, 22:35.
[5] PÄÄKKÖNEN V, VUORISTO J T, SALO T, et al. Comparative gene expression profile analysis between native human odontoblasts and pulp tissue[J]. Int Endod J, 2008, 41(2):117-127.
[6] CHEN C C, WEI X, LING J Q, et al. Expression of matrilin-2 and -4 in human dental pulps during dentin-pulp complex wound healing[J]. J Endod, 2011, 37(5):642-649.
[7] 谢金芳, 李晶, 李媛, 等. 细胞外基质Matrilin-4的研究进展[J]. 现代生物医学进展, 2017, 17(19):3795-3797.
[8] 杨楠, 李雯, 高华丽,等. Matrilin-2和Matrilin-4在大鼠牙髓损伤动物模型中的表达[J]. 现代口腔医学杂志, 2016,30(1):6-11.
[9] OPAL S, GARG S, DHINDSA A, et al. Minimally invasive clinical approach in indirect pulp therapy and healing of deep carious lesions[J]. J Clin Pediatr Dent, 2014, 38(3):185-192.
[10] HANADA K, MOROTOMI T, WASHIO A,et al.In vitro and in vivo effects of a novel bioactive glass-based cement used as a direct pulp capping agent[J]. J Biomed Mater Res Part B Appl Biomater, 2019, 107(1):161-168.
[11] LI Z F, CAO L H, FAN M W, et al. Direct pulp capping with calcium hydroxide or mineral trioxide aggregate:A meta-analysis[J]. J Endod, 2015, 41(9):1412-1417.
[12] LIKITPONGPIPAT N, SANGMANEEDET S, KLANRIT P, et al. Promotion of dental pulp wound healing in New Zealand white rabbits' teeth by thai propolis product[J]. J Vet Dent, 2019,36(1):17-24.
[13] SANGWAN P, SANGWAN A, DUHAN J, et al. Tertiary dentinogenesis with calcium hydroxide:A review of proposed mechanisms[J]. Int Endod J, 2013, 46(1):3-19.
[14] COLOMBO J S, MOORE A N, HARTGERINK J D, et al. Scaffolds to control inflammation and facilitate dental pulp regeneration[J]. J Endod, 2014, 40(4 Suppl):S6-12.
[15] TAHIRA T, JOUHAR R, GHANI H, et al. The effect of mineral trioxide aggregate as a direct pulp capping agent in permanent teeth[J]. Int J Oral Sci, 2018,10(6):310-313.
[16] BELOBROV I, PARASHOS P. Treatment of tooth discoloration after the use of white mineral trioxide aggregate[J]. J Endod, 2011, 37(7):1017-1020.
[17] ZHU L X, YANG J W, ZHANG J, et al. A comparative study of BioAggregate and ProRoot MTA on adhesion,migration, and attachment of human dental pulp cells[J]. J Endod, 2014, 40(8):1118-1123.
[18] LEAL F, DE-DEUS G, BRANDÃO C, et al. Similar sealability between bioceramic putty ready-to-use repair cement and white MTA[J]. Braz Dent J, 2013, 24(4):362-366.
[19] RAMOS J C, PALMA P J, NASCIMENTO R, et al. 1-year in vitro evaluation of tooth discoloration induced by 2 calcium silicate-based cements[J]. J Endod, 2016, 42(9):1403-1407.
[20] SHOKOUHINEJAD N, NEKOOFAR M H, PIRMOAZEN S, et al. Evaluation and comparison of occurrence of tooth discoloration after the application of various calcium silicate-based cements:An ex vivo study[J]. J Endod, 2016, 42(1):140-144.
[21] TIAN J, ZHANG YH, LAI ZH, et al. Ion release, microstructural, and biological properties of iroot BP plus and ProRoot MTA exposed to an acidic environment[J]. J Endod, 2017, 43(1):163-168.
[22] KIM J M, KANG S W, SHIN S M, et al. Inhibition of matrix metalloproteinases expression in human dental pulp cells by all-trans retinoic acid[J]. Int J Oral Sci, 2014, 6(3):150-153.

Matrilin-4对大鼠牙髓损伤后修复性牙本质形成的促进作用

Promotion effect of Matrilin-4 on reparative dentin formation after dental pulp injury in rats

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