吉林大学学报(医学版) ›› 2023, Vol. 49 ›› Issue (6): 1431-1436.doi: 10.13481/j.1671-587X.20230604

• 基础研究 • 上一篇    下一篇

头部针刺对局灶性脑缺血大鼠神经功能和脑组织中HIF-1α及VEGFR2表达的影响及其机制

周秀玲1,丛德毓2,张野1,张红石1()   

  1. 1.长春中医药大学护理学院,吉林 长春 130117
    2.吉林省中医院推拿科,吉林 长春 130021
  • 收稿日期:2023-02-16 出版日期:2023-11-28 发布日期:2023-12-22
  • 通讯作者: 张红石 E-mail:5503576@qq.com
  • 作者简介:周秀玲(1966-),女,河北省沧州市人,主任护师,医学学士,主要从事中西医结合医护理理论与实践方面的研究。
  • 基金资助:
    国家自然科学基金项目(82074569);国家重点研发计划项目(2018YFC1706002)

Effect of head acupuncture on neurological function and HIF-1α and VEGFR2 expressions in brain tissue in rats with focal cerebral ischemia and its mechanism

Xiuling ZHOU1,Deyu CONG2,Ye ZHANG1,Hongshi ZHANG1()   

  1. 1.School of Nursing,Changchun University of Traditional Chinese Medicine,Changchun 130117,China
    2.Department of Massage,Jilin Provincial Hospital of Traditional Chinese Medicine,Changchun 130021,China
  • Received:2023-02-16 Online:2023-11-28 Published:2023-12-22
  • Contact: Hongshi ZHANG E-mail:5503576@qq.com

摘要:

目的 探讨头部针刺对局灶性脑缺血大鼠神经功能和脑组织中缺氧诱导因子(HIF)-1α及血管内皮生长因子受体2(VEGFR2)表达的影响,并阐明其相关机制。 方法 采用改良大脑中动脉栓塞法(MCAO)制备大鼠局灶性脑缺血模型。成功制备模型的20只大鼠随机分为模型组和治疗组,每组10只;同时另取10只SD大鼠作为假手术组。治疗组大鼠进行头部针刺处理。记录各组大鼠神经功能评分并验证模型。Morris水迷宫试验检测各组大鼠学习记忆功能,酶联免疫吸附试验(ELISA)法检测各组大鼠血清和脑组织中HIF-1α水平,Western blotting法检测各组大鼠脑组织中HIF-1α和VEGFR2蛋白表达水平。 结果 神经功能评分,假手术组大鼠无神经功能缺陷;与假手术组比较,模型组和治疗组大鼠神经功能评分升高(P<0.05)。Morris水迷宫实验,与假手术组比较,模型组大鼠寻找平台时间增加(P<0.05);与模型组比较,治疗组大鼠寻找平台时间减少(P<0.05)。ELISA法检测,与假手术组比较,模型组大鼠血清和脑组织中HIF-1α水平均升高(P<0.05);与模型组比较,治疗组大鼠血清和脑组织中HIF-1α水平均升高(P<0.05)。Western blotting法检测,与假手术组比较,模型组大鼠脑组织中HIF-1α和VEGFR2蛋白表达水平均升高(P<0.05);与模型组比较,治疗组大鼠脑组织中HIF-1α和VEGFR2蛋白表达水平均升高(P<0.05)。 结论 头部针刺可修复局灶性脑缺血大鼠脑组织功能并调控大鼠脑组织中HIF-1α和VEGFR2水平升高,其相关机制可能与激活HIF/VEGFR2血管再生通路。

关键词: 头部针刺, 缺氧诱导因子, 血管内皮生长因子受体2, 局灶性脑缺血, 缺血性脑卒中

Abstract:

Objective To discuss the effect of head acupuncture on the neurological function and the expressions of hypoxia-inducible factor (HIF)-1α and vascular endothelial growth factor receptor 2 (VEGFR2) in the brain tissue of rats with focal cerebral ischemia, and to clarify the related mechanism. Methods The modified middle cerebral artery occlusion(MCAO) method was used to prepare the focal cerebral ischemia rat models.Twenty successfully modeling rats were randomly divided into model group and treatment group, and there were 10 rats in each group;another 10 SD rats were selected as sham operation group.The rats in treatment group were treated with scalp acupuncture. The neurological function scores of the rats in various groups were recorded and the model was verified; Morris water maze test was used to detect the learning and memory functions of the rats in various groups; enzyme-linked immunosorbent assay (ELISA) method was used to detect the level of HIF-1α in serum and brain tissue of the rats in various groups;Western blotting method was used to detect the expression levels of HIF-1α and VEGFR2 proteins in brain tissue of the rats in various groups. Results The neurological function scores detection results showed that there was no neurological deficit of the rats in sham operation group; compared with sham operation group, the neurological function scores of the rats in model group and treatment group were increased (P<0.05). The Morris water maze test results showed that compared with sham operation group, the time to find the platform of the rats in model group was increased (P<0.05); compared with model group, the time to find the platform of the rats in treatment group was decreased (P<0.05). The ELISA results showed that compared with sham operation group, the levels of HIF-1α in serum and brain tissue of the rats in model group were increased (P<0.05);compared with model group, the levels of HIF-1α in serum and brain tissue of the rats in treatment group were increased (P<0.05). The Western blotting results showed that compared with sham operation group, the expression levels of HIF-1α and VEGFR2 proteins in brain tissue of the rats in model group were increased (P<0.05); compared with model group, the expression levels of HIF-1α and VEGFR2 proteins in brain tissue of the rats in treatment group were increased (P<0.05). Conclusion The head acupuncture may restore the function of brain tissue in the rats with focal cerebral ischemia and up-regulate the levels of HIF-1α and VEGFR2 in brain tissue of the rats, and its related mechanism may be related to activating the HIF/VEGFR2 angiogenesis pathway.

Key words: Head needle, Hypoxia-inducing factor, Vascular endothelial growth factor receptor 2, Focal cerebral ischemia, Ischemic stroke

中图分类号: 

  • R246