吉林大学学报(医学版)

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PCSK9基因rs562556多态性与急性心肌梗死患者冠状动脉狭窄程度的关联性分析

刘园园1,才奇博1,曲妍1(),杨秀静1,关荣春1,刘灿君2   

  1. 1.齐齐哈尔医学院附属第三医院检验科,黑龙江 齐齐哈尔 161000
    2.齐齐哈尔医学院附属第三医院心血管科,黑龙江 齐齐哈尔 161000
  • 收稿日期:2023-11-02 出版日期:2024-11-22 发布日期:2024-11-22
  • 通讯作者: 曲妍 E-mail:liuyuany2023@163.com
  • 作者简介:刘园园(1982-),女,河南省周口市人,主管技师,医学硕士,主要从事医学检验基础和临床方面的研究。
  • 基金资助:
    黑龙江省卫健委科研项目(20210303010303)

Association analysis on rs562556 polymorphism of PCSK9 gene and degree of coronary artery stenosis of patients with acute myocardial infarction

Yuanyuan LIU1,Qibo CAI1,Yan QU1(),Xiujin YANG1,Rongchun GUAN1,Chanjun LIU2   

  1. 1.Department of Laboratory,Third Affiliated Hospital,Qiqihar Medical College,Qiqihar 161000,China
    2.Department of Cardiovascular Medicine,Third Affiliated Hospital,Qiqihar Medical College,Qiqihar 161000,China
  • Received:2023-11-02 Online:2024-11-22 Published:2024-11-22
  • Contact: Yan QU E-mail:liuyuany2023@163.com

摘要:

目的 分析急性心肌梗死(AMI)患者蛋白转化酶枯草杆菌素/酶切蛋白9型(PCSK9)基因rs562556多态性与冠状动脉狭窄程度的关联性。 方法 选取2021年1月—2022年12月确诊为AMI患者200例(AMI组),选取同期200例健康体检者作为对照组。根据Gensini评分标准将AMI组患者按照其病变程度分为轻危组(Gensini积分≤40分,n=78)和中高危组(Gensini积分>40分,n=122)。全自动生化仪检测2组研究对象血清中脂质代谢指标水平,酶联免疫吸附试验(ELISA)法检测2组研究对象血清中PCSK9水平,紫外分光光度法检测2组研究对象PCSK9基因单核苷酸基因多态性(SNP),Spearman相关性分析PCSK9基因rs562556多态性与疾病严重程度和患者血清中脂质代谢指标水平的相关性。 结果 与对照组比较,AMI组吸烟患者百分率明显升高(P<0.01)。与对照组比较,AMI组患者血清中低密度脂蛋白胆固醇(LDL-c)和PCSK9水平均明显升高(P<0.05);与轻危组比较,中高危组AMI患者血清中LDL-c和PCSK9水平均明显升高(P<0.05)。对照组和AMI组研究对象PCSK9基因rs1800487基因型分布符合Hardy-Weinberg(H-W)遗传平衡(χ2 =0.677,P=0.713;χ2 =0.970,P=0.831),与对照组比较,AMI组患者血清中PCSK9基因rs562556基因型AA与等位基因A分布频率明显升高(P<0.05)。轻危组和中高危组AMI患者PCSK9基因rs562556基因型分布符合H-W遗传平衡(χ2 =0.045,P=0.978;χ2 =1.290,P=0.525),与轻危组比较,中高危组AMI患者血清中PCSK9基因rs562556基因型AA与等位基因A分布频率明显升高(P<0.05)。PCSK9基因rs562556基因型AA与AMI严重程度(r=0.193,P=0.006)和LDL-c水平(r=0.301,P<0.01)呈正相关关系,等位基因A与LDL-c水平(r=0.168,P=0.017)呈正相关关系。 结论 PCSK9基因rs562556基因型AA与AMI患者冠状动脉狭窄程度呈正相关关系,其多态性可能通过上调LDL-c水平促进AMI疾病发展。

关键词: 急性心肌梗死, 基因多态性, 脂质代谢, 蛋白转化酶枯草杆菌素/酶切蛋白9型, 低密度脂蛋白胆固醇

Abstract:

Objective To analyze the association between the rs562556 polymorphism of the proprotein convertase subtilisin/kexin type 9(PCSK9) gene and the degree of coronary artery stenosis in the patients with acute myocardial infarction(AMI). Methods A total of 200 patients diagnosed with AMI from January 2021 to December 2022 were selected as AMI group, and 200 healthy individuals during the same period were selected as control group. According to the Gensini scoring standard, the patients in AMI group were divided into low risk group (Gensini score≤40, n=78) and medium-high risk group(Gensini score>40, n=122). The levels of lipid metabolism indicators in serum of the patients in two groups were detected by fully automatic biochemical analyzer; enzyme linked immunosorbent assay (ELISA) method was used to detect the PCSK9 levels in serum of the patients in two groups; ultraviolet spectrophotometry was used to detect the single nucleotide polymorphism (SNP) of PCSK9 gene of the patients in two groups; Spearman correlation analysis was used to detect the correlation between the rs562556 polymorphism of the PCSK9 gene and the degree of the disease and the levels of lipid metabolism indicators in serum of the patients. Results Compared with control group, the percentage of smokers of the patients in AMI group was significantly increased(P<0.01). Compared with control group, the levels of low-density lipoprotein cholesterol (LDL-c) and PCSK9 in serum of the patients in AMI group were significantly increased(P<0.05). Compared with low risk group, the levels of LDL-c and PCSK9 in serum of the patients in medium-high risk group were significantly increased(P<0.05). The distribution of PCSK9 gene rs1800487 genotype in both control and AMI groups conformed to the Hardy-Weinberg(H-W) equilibrium(χ2=0.677, P=0.713; χ2=0.970, P=0.831). Compared with control group, the distribution frequencies of PCSK9 gene rs562556 genotype AA and allele A of the patients in AMI group were significantly increased(P<0.05). In the AMI patients, the distribution of PCSK9 gene rs562556 genotype in both low risk and medium-high risk groups conformed to the H-W equilibrium(χ2=0.045, P=0.978; χ2=1.290, P=0.525). Compared with low risk group, the distribution frequencies of genotype AA and allele A of PCSK9 gene rs562556 of the patients in medium-high risk group were significantly increased(P<0.05). The PCSK9 gene rs562556 genotype AA was positively correlated with the degree of AMI(r=0.193, P=0.006) and LDL-c level(r=0.301, P<0.01). Allele A was positively correlated with the LDL-c level(r=0.168, P=0.017). Conclusion The PCSK9 gene rs562556 genotype AA is positively correlated with the degree of coronary artery stenosis of the AMI patients, and its polymorphism may promote the development of AMI by upregulating the LDL-c level.

Key words: Acute myocardial infarction, Gene polymorphism, Lipid metabolism, Proprotein convertase subtilisin/kexin type 9, Low-density lipoprotein cholesterol

中图分类号: 

  • R542.22