Abstract:

Abstract:Objective To study the inhibitory effect of triethyltin chloride (TETC) on proliferation  of rat C6 glioma cells in vitro and its mechanism and provide basis for research on TETC in treatment  for glioma.  Methods MTT assay was performed to determine the inhibitory rate of 0.5,1.0 and 2.0   μmol/L TETC on rat C6 glioma cells for 24  and 48 h.The  changes of nucleus of C6 glioam cells treated  with  0.5,1.0 and 2.0   μmol/L TETC for 48 h were observed by fluorescent microscope. Flow cytometry was used to assess the effects of 0.5,1.0 and 2.0   μmol/L TETC on cell cycle and apoptosis in rat C6 glioma cells for 48 h.  Results 0.5,1.0 and 2.0  μmol/L TETC  inhibited the proliferation of C6 glioma cells in vitro,and the inhibitory rates were 7.92%,9.51%,19.03% and 15.62%,36.16%,41.92% in   24 h TETC treated group and 48 h TETC treated group,respectively. The inhibitory rate of TETC on C6 glioma cells determined by MTT assay increased in a dose-dependent and time-dependent manner，and there were significant differences of the inhibitory rates at 48 h between  control and various doses groups as well as between various doses groups (P<0.05 or P<0.01) . Pyknotic nucleus and nuclear fragment were shown by fluorescent microscope. When C6 glioma cells were treated with 1.0 and 2.0  μmol/LTETC for 48 h,the ratio of G₀/G₁ phase cells significantly increased compared with control group(P<0.05);while the cells  at  S phase were decreased(P<0.05);the apoptotic cells were significantly higher than those in control group(P<0.05).
Conclusion TETC can inhibit the proliferation of rat C6 glioma cells in vitro,the mechanism of which may be involved in cell cycle arrest and apoptosis.

Key words: triethyltin compounds;glioma cells;apoptosis