N末端缺失萝卜谷胱甘肽磷脂氢过氧化物酶的表达、纯化及结构预测

Abstract

Abstract: An Nterminal 32 amino acids radish PHGPx gene (Δ32RsPHGPx) was cloned into expression vector pGEX-6P-1 and transformed into E.coli strain BL21 (DE3). Δ32RsPHGPx for crystallization was prepared via Glutathione SepharoseTM affinity, cationexchange and gel filtration chromatographies. Theconcentration of it was 10 g/L and the purity was over 95%. Δ32RsPHGPx showed obvious PHGPx activity towardslipid hydroperoxides. In addition, a tertiary structure model of Δ32RsPHGPx displayed the thioredoxin fold.

Key words: radish, phospholipid hydroperoxide glutathione peroxidase, expression, purification

CLC Number:

Q518.2

WANG Feng, LIU Jinyuan. Expression, Purification of a Recombinant NTerminal 32 Amino Acids Deletion Radish PHGPx[J].J4, 2008, 46(01): 148-153.

[1]	HU Huangshui, CAO Yu, LIU Mingyang, KANG Qi’er. Facial Expression Recognition Based on Local Correlation and Multi-scale Spatial Attention [J]. Journal of Jilin University Science Edition, 2026, 64(1): 104-0112.
[2]	HU Huangshui, QI Xingshuo, WANG Chuhang, WANG Ling. Multi-scale Facial Expression Recognition Method Based on Extended Local Binary Pattern [J]. Journal of Jilin University Science Edition, 2025, 63(5): 1427-1436.
[3]	GAO Gaofei, SHAO Dangguo, MA Lei, YI Sanli. Facial Expression Recognition Method Based on Lightweight Attention Residual Network [J]. Journal of Jilin University Science Edition, 2025, 63(2): 437-0444.
[4]	LIU Guixia, CAO Xintian, ZHAO He. Deep Learning Framework for Predicting Essential Proteins Based on Feature Graph Network and Multiple Biological Information [J]. Journal of Jilin University Science Edition, 2024, 62(3): 593-605.
[5]	GAO Yuqing, DONG Gangyin, ZHANG Hongrui, MA Zhanshan, FANG Li, ZHAN Dongling. Heterologous Expression and Property Characterization of Maltase-Glucoamylase D246A [J]. Journal of Jilin University Science Edition, 2024, 62(3): 742-749.
[6]	HONG Yong, ZHANG Lijuan, KONG Yinying, LI Zhen. Equivalent Conditions and Operator Expressions for the Best Half-Discrete Hilbert-Type Inverse Inequality with Non-homogeneous Kernel [J]. Journal of Jilin University Science Edition, 2023, 61(4): 823-830.
[7]	QI Miao, YAN Guangyou, XU Hui, SUN Hui. Facial Expression Recognition Based on Multi-scale Feature Selection Network [J]. Journal of Jilin University Science Edition, 2022, 60(2): 425-431.
[8]	CHEN Wenlong, ZHANG Yangyang, ZHANG Shengying, XING Xiaoyong, HU Yonghao. Cloning and Expression of E2 Protein of Bovine viral diarrheavirus and Preparation of Polyclonal Antibody [J]. Journal of Jilin University Science Edition, 2020, 58(3): 711-717.
[9]	LI Tingting, HU Yulong, WEI Fenglin. Improved Facial Expression Recognition AlgorithmBased on GAN and Application [J]. Journal of Jilin University Science Edition, 2020, 58(3): 605-610.
[10]	SHI Jingying, GUO Jing, LI Xue, DONG Xue, SUN Bo, WU Congmei, YIN Yuhe. Transient Expression of Antihuman PDL1 Monoclonal Antibodies [J]. Journal of Jilin University Science Edition, 2018, 56(4): 1024-1028.
[11]	JIN Hao, FU Lu, SHI Jingying, WU Congmei, WU Hui, YIN Yuhe. Expression, Purification and Immunogenicity of Recombinant Norovirus P Particles [J]. Journal of Jilin University Science Edition, 2017, 55(04): 1025-1030.
[12]	SUI Chunhong, XU Yawei, YAN Ganglin, LI Qiang,AN Ying, WANG Cheng. Preparation of a 76Mer SeleniumContaining Peptide with GPxand SOD Double Enzyme Activity and Its CellPenetrating Effects [J]. Journal of Jilin University Science Edition, 2017, 55(01): 168-174.
[13]	LIU Huimin, CHEN Fangqi, ZHENG Mingzhu, CHENG Guodong,ZHAN Dongling, LIU Jingsheng. Prokaryotic Expression and Characterization ofCysteine Protease from Zea mays [J]. Journal of Jilin University Science Edition, 2015, 53(05): 1054-1059.
[14]	LIU Hong, ZHANG Shugong. Facial Expression Synthesis Based on Curve Representation [J]. Journal of Jilin University Science Edition, 2015, 53(01): 80-88.
[15]	WU Ruihong, LV Xueqiang, LI Zhuo, SHU Yan. Extraction of Multiword Expressions in Questions ofQuestion Answering Communities [J]. Journal of Jilin University Science Edition, 2014, 52(06): 1230-1238.

Expression, Purification of a Recombinant NTerminal 32 Amino Acids Deletion Radish PHGPx

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10