Abstract: We investigated the molecular mechanism of Poria inhibition through network pharmacology and molecular docking technology, and verified the antibacterial effect of Poria in vitro through experiments. The results show that the antibacterial effect of Poria mainly relies on the regulation of tumor necrosis factor (TNF) and other targets and signaling pathways such as PI3K-Akt, and Poria extracts have inhibitory effect on the growth of Escherichia coli and Staphylococcus aureus, with the minimum inhibitory concentrations of 0.5,0.25 g/mL, respectively. The Poria contains triterpenoids such as oleanolic acid. The Poria is rich in polysaccharides that can exert antibacterial effect. The morphological structure of Staphylococcus aureus and Escherichia coli under the action of Poria is obviously damaged, and Poria can interfere with the synthesis process of the bacterial cell walls, and inhibit the formation of the cell membranes, thereby suppressing the growth of the bacterial strains.

Key words: Poria, antibacterial mechanism, Escherichia coli, Staphylococcus aureus