关键词: 成纤维细胞生长因子2, 流式细胞术, 方法, 细胞分离, 扩增

Abstract: Objective To study the growth pattern and surface makers of adult human mesenchymal stem cells (hMSC) and the influences of bFGF on the proliferation of hMSC. Methods The growth curves of the hMSC were drawn; FACS was use d to detect surface antigenic markers. Cell growth pattern and its response to bFGF were evaluated by MTT test. Results The latent phase of the primary culture cells was 5-9 days, followed by 11-16 days of logarithmical proliferation; the plateau began at day 18-20. The subcultures showed 12-24 hours of latent phase, followed by 7-10 days of logarithmical proliferation, then entered the plateau (11-13 days). The hMSC expressed positive CD44 unanimously, but don′t expressed CD45. Low concentration of bFGF (10 μg•L^-1) could stimulate the proliferation of hMSC significantly (P<0.05); while 25-100 μg•L^-1 of bFGF could inhibit the ALP activity. Conclusion hMSC can be expanded in vitro rapidly. The effect of bFGF on hMSC proliferation is in positive dose-effect manner.

Key words: fibroblast growth factor 2, flow cytometry, methods, cell separtion, amplification