还原型谷胱甘肽对糖尿病大鼠肾脏的保护作用及机制

doi:吉林省科技厅基金资助课题（990578）

还原型谷胱甘肽对糖尿病大鼠肾脏的保护作用及机制

罗健华^1,2，那宇²，张晓暄²，李银辉²

1. 解放军总医院肾科解放军肾病中心暨重点实验室，北京100853；2. 吉林大学第四医院肾内科，吉林长春130012

收稿日期:2005-11-25 修回日期:1900-01-01 出版日期:2006-03-28 发布日期:2006-03-28

Renoprotective effect of reduced glutathionein diabetic rats and its mechanism

LUO Jian-hua^1,2, NA Yu², ZHANG Xiao-xuan², LI Yin-hui²

1. Department of Nephrology, Kidney Center and Key Lab of PLA, General Hospital of PLA,Beijing 100853, China; 2. Department of Nephrology,Fourth Hospital，Jilin University, Changchun 130012,China

Received:2005-11-25 Revised:1900-01-01 Online:2006-03-28 Published:2006-03-28

摘要/Abstract

摘要： 目的：探讨还原型谷胱甘肽(Glut)对糖尿病大鼠肾脏的保护作用及可能机制。方法：以链脲佐菌素诱导糖尿病大鼠模型，同时设对照组，给予还原型谷胱甘肽、盐酸氨基胍(AG)单独或联合治疗8周，分别以RT-PCR及免疫组化法检测肾皮质TGF-β1 mRNA及蛋白表达，行肾组织PAS染色测定平均肾小球截面积(MGA)及体积(MGV)，同时检测尿白蛋白排泄率(UAER)、糖化血红蛋白(HbALC)、肾小球滤过率(Ccr)及肾重/体重比值。结果：8周时，各组糖尿病大鼠UAER、MGA、MGV、Ccr、肾重/体重比值、TGF-β1 mRNA及蛋白表达均较空白对照组明显增加(P<0.05或P<0.01)。还原型谷胱甘肽、盐酸氨基胍单独或联合治疗组UAER、MGA、MGV、Ccr、肾重/体重比值、TGF-β1 mRNA及蛋白表达均较糖尿病对照组明显降低(P<0.05或P<0.01)。结论：还原型谷胱甘肽对糖尿病大鼠肾脏具有保护作用。

关键词: 还原型谷胱甘肽, 氨基胍, 转化生长因子β1

Abstract: Objective To investigate the renoprotective effect of reduced glutathione on streptozotocin-induced diabetic rats and its possible mechanism. Methods Streptozotocin-induced diabetic rats received aminoguanidine 50 mg·kg^-1·d^-1 and reduced glutathione 400 mg·kg^-1·d^-1 intraperitoneally respectively or synchronously for 8 weeks. The expression of TGF-β1 mRNA and protein in renal cortex were determined by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry respectively. The mean glomerular area (MGA) and volume(MGV) were measured by image analysis system. The changes of creatinine clearance rate(Ccr),the kidney weight/body weight ratio and the urinary albumin excretion rate(UAER) were determined. Results By the end of 8 weeks, the Ccr, UAER, MGA, MGV, kidney weight/body weight ratio, the contents of TGF-β1 mRNA in renal cortex were increased significantly in DM groups compared with the blank control group (P<0.05 or P<0.01), and they were decreased significantly in aminoguanidine and reduced glutathione respectively or synchronously treatment groups compared with DM control group (P<0.05 or P<0.01). Conclusion Reduced glutathione has renoprotective effect on streptozotocin-induced diabetic rats, which may be at least partly correlated with suppression effect on increased oxidative stress as well as overexpression of TGF-β1 in renal tissue.

Key words: reduced glutathione, aminoguanidine, transforming growth factor-β1

中图分类号:

R587.1

罗健华，那宇，张晓暄，李银辉. 还原型谷胱甘肽对糖尿病大鼠肾脏的保护作用及机制[J]. J4, 2006, 32(2): 282-285.

LUO Jian-hua, NA Yu, ZHANG Xiao-xuan, LI Yin-hui. Renoprotective effect of reduced glutathionein diabetic rats and its mechanism[J]. J4, 2006, 32(2): 282-285.

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