关键词: p21^WAF1, CIP1<, sup>, HepG2, 转染

Abstract: Abstract:Objective To transfect a recombinant pcDNA3.1-DCN into HepG2 cells and detect the expressions of decorin(DCN) and p21^WAF1/CIP1 so as to investigate the mechanism of tumor suppression of DCN.Methods HepG2 cells were divided into pcDNA3.1-dec-HepG2 group(transfected group) and pcDNA3.1-HepG2 group (control group).After the recombinant pcDNA3.1-DCN and pcDNA3.1 were transfected into HepG2 hepatoma cells by liposome,the stably transfected cell lines were established using G418 screening. RT-PCR method was used to detect the expressions of DCN and p21^WAF1/CIP1 mRNA; Western blotting method was used to detect the expressions of DCN and p21^WAF1/CIP1 protein; immunohistochemistry was performed to detect the expression of DCN protein.Results Compared with control group,the expressions of DCN and p21^WAF1/CIP1 mRNA were significantly increased in transfected group by RT-PCR (P<0.05), the expressions of DCN and p21^WAF1/CIP1 protein increased obviously in transfected group by Western blotting (P<0.05),the expression of DCN protein increased in transfected group by immunohistochemistry (P<0.05). Conclusion The stably transfected HepG2 cell lines with DCN is successfully established and it is proved that DCN can increase the mRNA and protein expressions of p21^WAF1/CIP1.

Key words: p21^WAF1, CIP1<, sup>, HepG2, transfection