关键词: 高糖, 线粒体, 自由基, 电镜观察

Abstract: Abstract:Objective To explore the protective effects of Ginkgo biloba extract （GBE）on ECV304 cells exposed to high-level glucose and its mechanisms . Methods ECV304 cells were divided into four groups:high-level glucose group,GBE protective group,mannitol high-osmosis group and normal group. In vitro,ECV 304 cells were exposed to 35 mmol•L^-1 glucose for 24 h. The cells from different groups were collected,the production of super anion(O^-₂), hydroxyl radical(•OH)，level of malonaldehyde ( MDA),and activity of superoxide dismutase(SOD) were determined. The structures of mitochondria of cells in different groups were also observed under electronic microscope. Results The level of MDA and the production of O^-₂,•OH in ECV304 cells in high-level glucose group were significantly higher than those in GBE group（P<0.05）,the SOD activity was significantly lower than that in GBE group（P<0.05）. The level of MDA and production of O^-₂，•OH in mannitol group were lower than those in high-level glucose group(P<0.05).Under electronic microscope,the mitochondrial structure of ECV 304 cells in GBE group was normal and cristea were clear and in perfect shape,but the mitochondrial structure of ECV 304 cells in high-level glucose group was swollen and cristea were disappeared. Conclusion GBE plays a protective role in ECV 304 cells exposed to high-level glucose.

Key words: high glucose, mitochondria, free radicals, electronic microscope observation