宫颈癌患者血清和癌组织中NF-κB及TNF-α蛋白的表达及其意义

doi:10.7694/jldxyxb20130427

吉林大学学报(医学版)

宫颈癌患者血清和癌组织中NF-κB及TNF-α蛋白的表达及其意义

白雪^1,2，黄可欣¹，马洪喜³，李锐⁴，黄可敬⁵

（1. 吉林大学白求恩医学院形态学实验中心，吉林长春 130021；2. 中国人民解放军第202医院优生优育科，辽宁沈阳110003；3.吉林大学第一医院病理科，吉林长春 130021；4. 吉林省人民医院病理科，吉林长春130021；5.吉林省长春市中心血站，吉林长春130033）

收稿日期:2012-10-30 出版日期:2013-07-28 发布日期:2013-08-17
通讯作者: 马洪喜（Tel:0431-85619734,E-mail:497041338@qq.com） E-mail:497041338@qq.com
作者简介:白雪（1978-），女，辽宁省沈阳市人，主治医师，医学硕士，主要从事妇科肿瘤方面的研究。
基金资助:
吉林省教育厅科研基金资助课题（2012460）

Expressions of NF-κB and TNF-α protein in serum and carcinoma tissue of patients with cervical cancer

BAI Xue ^1,2,HUANG Ke-xin¹,MA Hong-xi³,LI Rui⁴,HUANG Ke-jing⁵

(1.Experiment Center of Morphology,Norman Bethune College of Medicine, Jilin University,Changchun 130021,China;2. Depertment of Eugenics Branch,No. 202 Hospital of PLA,Shenyang 110003,China;3. Department of Pathology,First Hospital,Jilin University,Changchun 130021,China;4. Department of Pathology, People’s Hospital of Jilin Province,Changchun 130021，China;5. Blood Bank of Changchun City,Jilin Province, Changchun 130033，China)

Received:2012-10-30 Online:2013-07-28 Published:2013-08-17

摘要/Abstract

摘要：

目的：探讨不同宫颈病变患者血清和宫颈组织中核因子（NF-κB）及肿瘤坏死因子α（TNF-α）蛋白的表达，阐明其生物学意义和早期联合诊断的价值。方法：对100例有宫颈癌病变高危因素的患者（宫颈炎37例、宫颈内瘤变39例和宫颈癌24例）抽取静脉血同时行阴道镜检。采用ELISA方法检测血清及镜检宫颈组织中NF-κB和TNF-α蛋白的含量；采用RT-PCR法检测宫颈组织中NF-κB及TNF-α的mRNA表达水平；采用免疫组织化学方法检测宫颈组织中NF-κB及TNF-α的蛋白表达；结合临床病理特点进行分析。结果：宫颈内瘤变及宫颈癌患者血清及镜检组织中NF-κB和TNF-α含量明显高于宫颈炎患者（P<0.01），宫颈癌患者其含量明显高于宫颈内瘤变患者（P<0.01）；宫颈内瘤变和宫颈癌患者宫颈组织中NF-κB和TNF-α mRNA及其蛋白表达水平明显高于宫颈炎患者（P<0.01），且宫颈癌患者宫颈组织中其表达水平明显高于宫颈内瘤变患者（P<0.01）。宫颈炎、宫颈内瘤变及宫颈癌患者宫颈组织中NF-κB与TNF-α含量呈正相关关系（r=0.69，P＜0.05）。结论：NF-κB和TNF-α均可独立作为早期诊断宫颈癌前病变及宫颈癌的标记物，二者的正相关性使得联合检测可提高宫颈癌的早期诊断率。

关键词: 子宫颈肿瘤, 宫颈癌前病变, 细胞核因子, 肿瘤坏死因子

Abstract:

Objective To explore the expressions of nuclear factor(NF-κB ) and tumor necrosis factor(TNF-α) protein in serum and carcinoma tissue of the pati
ents with cervical cancer,and to clarify their biological significance and the values in early combined diagnosis.Methods The blood samples were collected and colposcope was performed in 100 patients with high-risk factors of cervical lesion (37 cases of cervicitis,39 cases of cervical intraepithlial neoplasia,24 cases of cervical cancer).The ELISA method was used to detect the contents of NF-κB and TNF-α protein in the serum and cervix tissue.The expression levels of NF-κB and TNF-α mRNA in cervix tissue were determined with RT-PCR assa.The immunohistochemical method was used to detect the expressions of NF-κB and TNF-α protein in cervix tissue;the relationship with clinical and pathological features was analyzed.Results The contents of NF-κB and TNF-α protein in serum and cervix tissue in the patients with cervical intraepithelial neoplasia and cervical cancer were significantly higher than those in the patients with cervicitis(P<0.01).The contents of NF-κB and TNF-α protein in the patients with cervical cancer were significantly higher than those in the patients with cervical intraepithelial neoplasia(P<0.01).The NF-κB and TNF-α mRNA expression levels and protein levels in the cervix tissue of the patients with cervicitis were significantly lower than those of the patients with cervical neoplasia and cervical cancer(P<0.01),and the expression levelsNF-κB and TNF-α mRNA and NF-κB and TNF-α protein in cervical tissue of the patients with cervical intraepithelial neoplasia were significantly lower than those of the patients with cervical cancer(P<0.01).The expressions of mRNA and protein of NF-κB and TNF-α showed positive correlation between the patients with cervicitis,cervical intraepithelial neoplasia and cervical cancer(r=0.69，P=0.037).Conclusion NF-κB and TNF-α can be independent as markers for early diagnosis of cervical precancerous lesions and cervical cancer,and the positive correlation between them can make the combined detection to improve the early diagnosis rate of cervical cancer.

Key words: cervix neoplasms, cervical precancerous lesions, nuclear factor, tumor necrosis factor

中图分类号:

白雪，黄可欣，马洪喜，李锐，黄可敬. 宫颈癌患者血清和癌组织中NF-κB及TNF-α蛋白的表达及其意义[J]. 吉林大学学报(医学版), 2013, 39(4): 773-776.

BAI Xue,HUANG Ke-xin,MA Hong-xi,LI Rui,HUANG Ke-jing. Expressions of NF-κB and TNF-α protein in serum and carcinoma tissue of patients with cervical cancer[J]. Journal of Jilin University Medicine Edition, 2013, 39(4): 773-776.

[1]	曹耿飞,沙娅·玛哈提null,顾俊鹏,纪卫政,阿斯哈尔·哈斯木null,任伟新. DEB-TACE与c-TACE治疗中晚期肝细胞癌的免疫微环境差异及TRADD介导Th17分化的机制[J]. 吉林大学学报(医学版), 2026, 52(3): 764-780.
[2]	秦迪,黄丽红,郑庆霜,孙静静,徐为民. 老年肌少症肥胖伴糖尿病患者血清中促炎因子与肌肉质量的关联性分析[J]. 吉林大学学报(医学版), 2025, 51(5): 1293-1302.
[3]	仲威,戴芝银,崔星钢,李波,姜瑜. 活化T淋巴细胞核因子5在高盐诱导小鼠平滑肌细胞衰老中的作用及其机制[J]. 吉林大学学报(医学版), 2025, 51(3): 567-575.
[4]	李洪杰,兰茂卓,王潇,冯冉冉,陶燕燕,刘佳庆,孙海柏. BTK抑制剂BGB-3111联合硼替佐米对人多发性骨髓瘤细胞凋亡的影响及其机制[J]. 吉林大学学报(医学版), 2025, 51(3): 599-609.
[5]	张京顺,邹颖刚,郑连文. 过表达SLC7A5基因对大鼠卵巢颗粒细胞凋亡的影响及其机制[J]. 吉林大学学报(医学版), 2024, 50(6): 1526-1534.
[6]	宋传龙,焦红杰,海力其古丽·努日丁null,岳迎宾,严媚. 包虫抗原B通过肿瘤坏死因子受体2调控巨噬细胞极化对小鼠免疫性血小板减少症的改善作用[J]. 吉林大学学报(医学版), 2023, 49(4): 858-866.
[7]	林喆,王泰栋,黄晓巍,马超,庄雪峰,王雨辰,林贺,郭骏骐,律广富. 防风野生品对大鼠类风湿性关节炎的改善作用及其机制[J]. 吉林大学学报(医学版), 2023, 49(3): 617-624.
[8]	林雄,瞿领航,许静,刘春莲,李水清,刘艳菊. 苍术挥发油和苍术醇提物对溃疡性结肠炎模型小鼠的改善作用及其效果比较[J]. 吉林大学学报(医学版), 2022, 48(5): 1247-1255.
[9]	王琴, 林春霖, 程志彬, 何若凡, 林鹏航, 陈辉, 叶建新, 朱广伟. 肿瘤坏死因子受体相关因子6泛素化位点突变载体的构建及其功能位泛素化位点的鉴定[J]. 吉林大学学报(医学版), 2021, 47(3): 551-558.
[10]	李慧,李明贺,赵聪,苏畅. 盐酸米诺环素治疗绝经妇女牙周炎患者的疗效及其对龈沟液中炎性因子和骨代谢因子的影响[J]. 吉林大学学报(医学版), 2020, 46(6): 1304-1308.
[11]	宋续军, 张倩, 王少磊, 柏娜, 刘杰. 实验性种植体周围炎小鼠模型的建立及TNF-α在模型小鼠牙龈组织中的表达[J]. 吉林大学学报(医学版), 2020, 46(01): 40-44.
[12]	华进, 程志彬, 林春霖, 戴起宝, 朱广伟. 一种高效稳定的磷酸钙转染293T细胞方法的建立及评价[J]. 吉林大学学报(医学版), 2019, 45(05): 1177-1181.
[13]	牛想娥, 张琦, 余静, 蔺文燕, 刘静. 补体C1q/肿瘤坏死因子相关蛋白13的研究进展[J]. 吉林大学学报(医学版), 2019, 45(05): 1188-1192.
[14]	王叶, 王红, 张书剑, 姬华祎, 金正勇. 谷氨酰胺对新生大鼠高氧肺损伤的干预作用及其机制[J]. 吉林大学学报(医学版), 2019, 45(04): 747-751.
[15]	廖鑫, 邓凡曲, 宋美潓, 高琳, 张晗, 张琳, 王雪梅, 章莹, 赵宇. 不同糖代谢状态人群血清CTRP3和25(OH) D水平及其与胰岛素抵抗的关系[J]. 吉林大学学报(医学版), 2019, 45(04): 887-892.

宫颈癌患者血清和癌组织中NF-κB及TNF-α蛋白的表达及其意义

Expressions of NF-κB and TNF-α protein in serum and carcinoma tissue of patients with cervical cancer

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10