吉林大学学报(医学版) ›› 2013, Vol. 39 ›› Issue (2): 318-321.doi: 10.7694/jldxyxb20130228

• 基础研究 • 上一篇    下一篇

miR-21在结肠癌与癌旁组织中的表达及其临床意义

鲍 欣1，杨艳明1，刘 念1， 尹光浩1， 孟凡旭1，程 颖2，刘林林1   

  1. 1. 吉林大学第二医院肿瘤生物治疗中心，吉林 长春 130041； 2. 吉林省肿瘤医院，吉林 长春 130012
  • 收稿日期:2012-10-31 出版日期:2013-03-28 发布日期:2012-03-28
  • 通讯作者: 刘林林 （Tel:0431-88796640,E-mail:liulinlin@jlu.edu.cn） E-mail:liulinlin@jlu.edu.cn
  • 作者简介:鲍 欣（1986-），女，吉林省吉林市人，在读医学硕士，主要从事 肿瘤生物治疗方面的研究。
  • 基金资助:

    中国博士后基金项目资助课题(20060390906)

Expressions of miR-21 in colon cancer and cancer-adjacent
colorectal tissues and their clinical significances

BAO Xin1，YANG Yan-ming1，LIU Nian1，YIN Guang-hao1，MENG Fan-xu1，CHENG Ying2,LIU Lin-lin1   

  1. 1. Cancer Biotherapy Center,Second Hospital,Jilin University,Changchun 1300
    41,China;2.Jilin Provincial Tumor Hospital，Changchun 130012，China
  • Received:2012-10-31 Online:2013-03-28 Published:2012-03-28

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摘要： 目的：检测MicroRNA-21（miR-21）在结肠癌及其相应癌旁正常组织中的表达，探讨其与结肠癌临床病理指标的关系。方法： 应用实时荧光定量PCR（RT-qPCR）技术检测50例结肠癌组织标本和癌旁组织标本中miR-21的相对表达量，分析其在2种组织中的表达变化，探讨miR-21表达水平与结肠癌发病位置、临床分期、浸润深度及有无淋巴结转移的关联性。结果：miR-21在结肠癌组织中相对表达水平显著高于癌旁组织(P<0.05)，右半结肠癌组织中miR-21表达水平明显高于左半结肠癌组织 (P<0.05)；Ⅲ期和Ⅳ期结肠癌组织中miR-21表达水平明显高于Ⅰ期和Ⅱ期结肠癌(P<0.05)；miR-21表达水平在不同浸润深度结肠癌之间比较差异无统计学意义(P>0.05)；有淋巴结转移结肠癌组织中miR-21表达水平明显高于无淋巴结转移者(P<0.05)。结论：相对于癌旁组织，miR-21在结肠癌组织中表达明显上调，且表达水平与发病位置、临床分期及是否有淋巴结转移有关。

关键词: 微小RNAs, 结肠肿瘤, 聚合酶连反应

Abstract: Abstract:Objective  To detect the expression levels of MicroRNA-21 (miR-21) in the colon cancer tissue samples and the cancer-adjacent tissues,and to explorethe relationship between the clinicopathological features and the expression of miR-21.Methods Real-time florescence quantitative PCR（RT-qPCR） was used to detect the relative expression levels of miR-21 in 50 samples including the colon cancer tissue samples and the cancer-adjacent tissues；and the expression changes of miR-21 in two kinds of tissues were analyzed.The relationships between themiR-21 expression levels and the location,depth of invasion and lymph node metastasis of colon cancer were studied.Results The miR-21 relative expression level in colon cancer tissue samples was significantly higher than that in cancer-adjacent tissues(P<0.05);the miR-21 expression level in  right colon cancer tissues was obviously higher than that in left colon cancer tissues (P<0.05);the  miR-21 expression level in Ⅲ stage and Ⅳ stage colon cancer tissues was  obviously higher than  that in Ⅰ stage and Ⅱ stage colon cancer tissues(P< 0.05);the expression levels of MiR - 21 in colon cancer with different depths of invasion had no statistically significant differences(P>0.05);the expression level of miR-21 in  colon cancer tissues with lymph node metastasis was  significantly higher than that in colon cancer tissues without lymph node metastasis (P>0.05).Conclusion The miR-21 expression level is increased  significantlyin colon cancer tissues compared with  cancer-adjacent tissues,and it is  related to the location,the clinical stage and the lymphy node metastasis of colon cancer.

Key words: microRNAs, colon neoplasms, polymerase chain reaction

中图分类号: 

  • R735.3 
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