关键词: 金雀异黄素, 肝肿瘤,实验性, 细胞凋亡

Abstract:

To investigate the influence of genistein(Gen) on the growth  and apoptosis regulation of hepatoma carcinoma cells SMMC-7721.Methods SMMC-7721 cells were divided into three Gen groups treated with different concentrations(5.0,10.0,20.0 mg·L^-1）and control group(without Gen).After SMMC-7721 cells were treated for 24 and 48 h,the changes of ultrastructure of cells were observed under electron microscope, MTT was used to detect the inhibitory rate of proliferation of SMMC-7721 cells, flow cytometry was performed to analyze cell phase;immunohistochemistry was used to detect the expression of Caspases-3 protein.Results Compared　 with control group,chromatin in cellular nucleus became conglomeration or lumping,with chondriosome swelling in Gen groups. With the increasing of Gen concentration,the cellular nucleus became condensed,heterochromatin agglutinating,ground cytoplasm obviously cavitating;with the increase of Gen concentration and prolongation of  time,from 24 to 48 h, MTT showed the inhibitory rate of  proliferation  of SMMC-7721 cells  increased  in a time- and dose-dependent  manner.Flow cytometry analysis showed that with the increase of Gen concentration,the number of cells at G₂/M  phase increased,the number of cells at S phase decreased and the ratio of G₀/G₁ also decreased.Immunohistochemistry results showed the expression of protein Caspases-3 increased with the Gen concentration in a dose
-dependent manner（P<0.01）.
Conclusion Gen has significantly  inhibitory effect on the growth of hepatoma carcinoma cells SMMC-7721,up-regulating the expression of Caspases-3 protein and inducing  apoptosis may be one of the mechanisms.

Key words: genistein, liver neoplasms,experimental, apoptosis