Abstract:

Objective To study the influence of oxytocin(OT) in the proliferation and osteogenesis activity of MG-63 cells,and to clarify its function on bone metabolism.Methods The MG-63 cells were incubated with  10,50 and  100 nmol/LOT for 1,3,5 and 7 d. The cell proliferation was detected by MTT,then  the minimum effective concentration was used to  detect the change of alkaline phosphatase (ATP) activity .Results The  MTT test results showed that    the  proliferation  of MG-63 cells after treated with  50,100 nmol/L OT for 1,3,5 and 7 d was  significantly higher than those in control group(P<0.05);and  the proliferation of  MG-63  cells after treated with 10 nmol/LOT for 7 d was significantly higher than that in control group (P<0.05).The ELISA test results showed that the ALP  activities of MG-63 cells after treated with 50 nmol/L  OT  for 3,5 and 7 d were sifnificantly higher than those in control group(P<0.05).Conclusion OT has a regulation effect on proliferation of MG-63 cells,and it can promote the  proliferation and differiation of MG-63 cells.

Key words: oxytocin；alkaline phosphatase；cell proliferation;osteosarcoma cell line