Abstract:

Abstract:Objective  To investigate the effect of the expression of sodium ferulate(SF) on the related protein of Rho/Rock signal pathway and to clarify the protective effects on liver tissue and myocardium tissue.Methods Twenty male Wistar rats were divided into normal control group, model group, positive medicine group,and sodium ferulic group.Besides normal control group,the rats in the  other groups were injected with 15 mg/kg Iso to induce rat myocardial fibrosis.2 d later, except control and model groups, the rats in the other groups were given SF and Bena zepril.The  change of collagen content in myocardium was analyzed by Masson staining.The expressions of Rho A and Rock  mRNA were analyzed by RT-PCR.The expression of Rock  protein was analyzed by immunohistochemistry. The Raman spectrum was used to detect the liver tissue injury.Results Compared with control group,the expressions of RhoA and Rock mRNA were increased after injection with Iso for 3 weeks(P<0.01);compared with model group,the expressions of Rho A and Rock mRNA and protein were decreased after injection with SF (P<0.01).The Raman spectrum results showed that the peak value in SF group shifted to the right with one unit compared with model group,and tended to normal control group.Conclusion SF can not only protect the heart against fibrosis by inhibiting Rho/Rock pathway, but also can protect liver damage.

Key words: myocardial fibrosis, sodium ferulate, Rho/Rock pathway