Abstract:

Objective To study the expressions of galactose Galectin-3 and integrin ανβ3 mRNA in glioma cells  and their roles in the occurrence and the malignancy progress  of glioma.Methods  In situ hybridization was used to detect the expressions of Galectin-3 and integrin ανβ3 mRNA in 5 normal brain tissues and 40 patients with different grades gliomas , and the relationship between them was analyed. Results The expressions of Galectin-3 and integrin ανβ3 mRNA in normal brain tissue were negative.Galectin-3 mRNA mainly expressed in the cytoplasm and membrane of tumor cells.In 23 cases ofⅠ, Ⅱ grade glioma specimens, 2 cases were positive,4 cases were weakly positive and 17 cases were negative, the positive rate was 29.09%(6/23), LI value was (4.80±3.22)%;in  17 cases of Ⅲ, Ⅳ grade glioma specimens,4 cases were strongly positive,6 cases were positive ，1 case was weakly positive and 6 cases were negative，the positive rate was 64.71%(11/17), LI value was (27.30±22.72)%; there was significant difference in the positive rate betweenⅠ，Ⅱ grade and Ⅲ, Ⅳgrade glioma(P<0.05). The integrin ανβ3 mRNA mainly expressed in the cytoplasm and membrane of tumor cells. In 
23 cases of Ⅰ, Ⅱ grade glioma specimens 3 cases were positive expressin of integrin αυβ3,4 cases were weakly positive, 16 cases were negative, the positive rate was 34.43% (7 / 23), LI value was (5.35 ± 3.79)%; in  17 cases of Ⅲ, Ⅳ grade glioma specimens,4 cases were strongly positive, 6 cases were positive, 3 cases were weakly positive, and 4 cases were negative，the positive rate was 76.47% (13/17), and LI value was (29.24± 21.10)%; there was significant difference in the positive rate betweenⅠ，Ⅱ grade and Ⅲ, Ⅳgrade glioma(P<0.05).The linear correlation analysis showed that the expression rates of Galectin-3 and integrin ανβ3 mRNA  were positively correlated (P<0.05).Conclusion The expressions of Galectin-3 and integrin ανβ3 mRNA  in Ⅰ, Ⅱ grade gliomas are significantly lower than those of Ⅲ, Ⅳ grade glioma,which indicates that the detection of  Galectin-3 and integrin  ανβ3 mRNA  can be used as an indicator to judge malignant biological behavior of human brain glioma at molecular level.

Key words: glioma, Galectin-3, integrin ανβ3, in situ hybridization