异氟醚对Aβ25-35诱导大鼠PC12细胞氧化应激损伤的影响及海藻糖的保护作用

doi:10.13481/j.1671-587x.20150201

Abstract

Abstract:

Objective To explore the effect of isoflurane on the oxidative stress injury induced by beta-amyloid protein (Aβ) 25-35 in PC12 cells of the rats,and to clarify the possible protective effects of trehalose on this injury.Methods The PC12 cells of rats were randomly divided into normal control group (Control group,treated with normal cell culture medium),isoflurane group (Iso group,treated with 2% isoflurane),Aβ25-35 group(Aβ group,treated with 10 μmol·L^-1 Aβ25-35),isoflurane+Aβ25-35 group (Iso+Aβ group,treated with 2% isoflurane and 10 μmol·L^-1 Aβ25-35),isoflurane+Aβ25-35+trehalose group (Iso+Aβ+Tre group,treated with 2% isoflurane,10 μmol·L^-1 Aβ25-35 and 200 mmol·L^-1 trehalose),and trehalose group (Tre group,treated with 200 mmol·L^-1 trehalose).The survival rates of the PC12 cells were detected by MTT assay;the apoptotic rates of the PC12 cells in various groups were determined by Hoechst 33342 staining;the levels of intracellular reactive oxygen species (ROS) of the PC12 cells were measured by DCFH-DA fluorescence assay;the levels of malondialdehyd (MDA) and the activities of superoxide dismutase (SOD),glutathion peroxidase (GSH-Px), and catalase (CAT) of the PC12 cells were detected by commercial kits.Results Compared with control group,the apoptotic rates of the cells were increased and the survival rates were decreased,and the levels of ROS and MDA and the activities of SOD,GSH-Px, and CAT in the PC12 cells were significantly decreased (P<0.05 or P<0.01) in Aβ group,Iso group and Iso+Aβ group.Compared with Iso group or Aβ group,the apoptotic rates were increased,the survival rates were decreased,the levels of ROS and MDA were increased,and the activities of SOD,GSH-Px,and CAT in the PC12 cells in Iso+Aβ group were decreased(P<0.05).Compared with Iso+Aβ group,the survival rates were increased and the apoptotic rates were decreased and the levels of ROS and MDA were decreased,and the activities of SOD,GSH-Px,and CAT in the PC12 cells in Iso+Aβ+Tre group were increased (P<0.05).Conclusion Isoflurane could aggravate the oxidative stress injury and the apoptosis induced by Aβ25-35 in the PC12 cells of the rats,and trehalose could alleviate the cytotoxicity of isoflurane via inhibition of oxidation and apoptosis.

Key words: isoflurane, Alzheimer's disease, PC12 cells, oxidative stress, apoptosis, trehalose

CLC Number:

R614.24

XU Shanshan, PIAO Meihua, WANG Yanshu, LIU Nan, PEI Aiyue, FENG Chunsheng. Effect of isoflurane on oxidative stress injury induced by Aβ25-35 and protective effects of trehalose in PC12 cells of rats[J].Journal of Jilin University Medicine Edition, 2015, 41(02): 207-212.

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Effect of isoflurane on oxidative stress injury induced by Aβ25-35 and protective effects of trehalose in PC12 cells of rats

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