Abstract:

Abstract:Objective To observe the efficiency of vascular endothelial cell growth factor(VEGF) gene transferring into fibroblasts in vitro by diagnostic ultrasound combined contrast agent. Methods  The fibroblasts were divided into four groups:pure VEGF₁₆₅ plasmid,contrast agent and VEGF₁₆₅plasmid,ultrasound plus VEGF₁₆₅ plasmid (MI 1.6),ultrasound plus contrast agent and VEGF₁₆₅plasmid (MI 1.2,1.4,and 1.6). The transfection efficiencies of the VEGF gene in the fibroblasts were observed under confocal microscopy respectively in different groups.
Results There was no significant difference of transfection efficiency among ultrasound plus plasmid group,contrast agent plus plasmid group and pure plasmid group (P>0.05).The transfection efficiencies were improved significantly in the ultrasound plus contrast agent and VEGF plasmid group (MI 1.2,1.4,and 1.6) compared with pure plasmid group（P<0.01).The transfection efficiencies were higher in ultrasound plus contrast agent and VEGF₁₆₅ plasmid group (MI 1.4,1.6),but there was no significant difference between the two groups (P>0.05);there were significant differences between ultrasound plus contrast agent and VEGF₁₆₅plasmid(MI 1.4，1.6)　 and  ultrasound plus contrast agent and VEGF₁₆₅plasmid group (MI 1.2) (P<0.01).
Conclusion Ultrasound combined with contrast agent can improve the transfection efficiency of VEGF gene,and the transfection efficiency increases with the intensity of ultrasound.

Key words: ultrasound;contrast agent;vascular endothelial cell growth factor;transfection;fibroblast