Abstract:

Abstract:Objective To study the interference effect of garlic polysaccharide on alcoholic liver injury in mice  and clarify its protective effects and mechanism,and to  provide experimental basis for developing natural antioxidant medicine for prevention and treatment of alcoholic liver  disease liver .  　Methods 70　 Kunming mice were randomly divided into normal group,control group,model group,garlic polysaccharide low,medium and high-dose (100,150,and 200  mg•kg^-1•d^-1）groups, fourteen mice in each group．Dose increasing method was used to set mouse chronic alcoholism models with 56°wine,meanwhile,the experiment was intervened with garlic polysaccharide. Eight weeks later,the weight,liver weight index,the levels of hepatic(MDA) and glutathione(GSH) and the activities of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),sodium potassium pump (Na⁺-K⁺-ATP),glutathione peroxidase(GSH-Px),superoxide dismutase (SOD) were measured,and the morphological changes of liver tissue in mice were observed with　light microscope.  Results Compared　 with control group,the weights of the mice in  model group were markedly decreased (P<0.05),while the liver weight index,ALT and AST levels in serum,and MDA content in liver were all significantly increased (P<0.05)；the activities of  Na⁺-K⁺-ATP,GSH-Px and SOD and GSH level were evidently decreased (P<0.05). In addition,the pathological changes in liver appeared  fat vacuole,inflammatory cell infiltrate,etc. Compared with model group,in  garlic polysaccharide  groups,the liver weight indexes of mice and the levels of serum ALT and AST were significantly decreased (P<0.05),while the levels  of GSH were increased and the activities of GSH-Px and SOD were greatly increased (P<0.05);in medium and high dose groups,the weights and the activities of Na⁺-K⁺-ATP were significantly increased (P<0.05),while the levels of MDA were obviously decreased (P<0.05) and the degree of pathological changes in liver  tissue was reduced. Conclusion Unremitting superfluous drinking can lead mice to alcoholism,and cause serious damage to their livers. Garlic polysaccharide has certain protective effects on liver injury induced by alcohol in mice. The mechanism is relevant to its increasing anti-oxygen capacity of the organism.

Key words: garlic polysaccharide;alcoholic liver injury;antioxidation