Abstract:

Abstract:Objective  To observe the effect of Tongxinluo on the expreeions of plasminogen activator inhibitor type-1 (PAI-1) and vascular cell adhension molecule-1 (VCAM-1) in rabbits with atherosclerosis(AS),and explore the possible mechanism.Methods Twenty four rabbits were randomly divided into control,cholesterol diet,and Tongxinluo groups.The levels of serum cholesterol(TC),triacylglycerol(TG),low density lipoprotein(LDL),PAI-1 and VCAM-1 were measured before and after treatment.The aortas were harvested for pathologic morphological observation.Immunohistochemistry analysis was used to evaluate the positive percentages of  PAI-1 and VCAM-1.Results There were no differences of TC,TG,LDL,PAI-1 and VCAM-1 between three groups before treatment （P>0.05）. 14 weeks after treatment,the TC,TG,LDL,PAI-1 and VCAM-1 levels in cholesterol diet group and Tongxinluo group were significantly higher than those in control group(P<0.01),while the TC,TG,LDL,PAI-1and VCAM-1 levels in Tongxinluo groups were signficantly lower than those in cholesterol diet group(P<0.01). The expressions of  PAI-1 and VCAM-1 in aorta in cholesterol diet and Tongxinluo group were higher than those in control group(P<0.01),and the expressions of PAI-1 and VCAM-1     in Tongxinluo group  were lower than those in cholesterol group(P<0.01).Conclusion PAI-1and VCAM-1 over express   in   AS rabbits,while inhibiting the expressions of PAI-1 and VCAM is likely to be one of anti-AS  mechanism of  Tongxinluo.

Key words: Tongxinluo;atherosclerosis;plasminogen activator inhibitor 1;vascular cell adhesion molecule-1