Abstract:

Objective To isolate and purify hops flavonoids by ethanol extraction
 and macroporous resin adsorption,and to optimize process parameters accordin
g to content and yield of flavonoids,and to establish process system for large-
scale production of hops flavonoids.Methods In the ethanol extraction based on
the total flavonoids from Humulus lupulus,by static adsorption and elution method of

macroporous resin the total flavonoids of hops were separated and purified.According to leaching effect and

the rates of adsorption and elution, the optimal process parameters were determined.
Results The optimal procedure was established,and the ethanol concentration was 40%;FL-3 macroporous resin

was applied on the condition that ethanol concentration was 20% and pH value of leacheate was 4 with
the absorption and elution experiment of static state,then the condition was changes,the condition of ethanol concentration 60% and pH value of leacheate 7 w
as used and eluted.The recovery rate of  total flavonoids from hops was 70.2%,and the
 purity quotient was 70.6%.Conclusion Ethanol extraction method combined with 
FL-3 macroporous resin adsorption can achieve the purification of flavonoids from hops.

This process has the advantages of simple operation and a large amount of
 sample processing,and it is suitable for large scale production.
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Key words: hops, flavonoids, purification, macroporous resin, static adsorption