Abstract:

Objective To investigate the expressions of Livin and cytochrome C( Cyt-C) in tongue squamous cell carcinoma (TSCC) tissue and to
 explore the correlation between their expression levels and  the   clinicaopathological characteristics of TSCC and the correlation between expressions of Livin an
d Cyt-C protein in TSCC tissue. Methods The expressions of Livin and Cyt-C protein were examined by immunohistochemistry SP method in 48 cases of TSCC and 10 casesof normal tongue tissue,and  the relationship between the expressions of Livin,Cyt-C protein and the   clinicopathological characteristics,such as gender,age,histological type and lymph node metastasis were analyzed. Results Livin mainly expressed   in the cytoplasm. The pos
itive expression rates of  Livin protein in TSCC and normal tongue tissue were 66.67% and 0%,respectively,and there was significant difference  (P<0.01).The  high
 
expression of Livin was correlated to the histological differentiation degree of TSCC (P<0.05),and also closely correlated to the cervical lymph node metastasis (P<0.05). Cyt-C  mainly expressed   in the cytoplasm. The positive expression rates of Cyt-C protein in TSCC and normal tongue tissue were 70.83% and 10.00%,respectively,and there was  significant difference (P<0.01).The  expression of Cyt-C was correlated to the histological differentiation of TSCC (P<0.05).The Spearman analysis results showed that  there was a positive correlation between the expressions of Livin and Cyt-C protein  in TSCC tissue(r=0.553,P<0.001). Conclusion There is a positive correlation between the expressions of Livin and Cyt-C protein in TSCC tissue. Combined detection of livin and Cyt-C will help to judge the  malignant degre
e of TSCC and evaluate the  prognosis.

Key words: tongue squamous cell carcinoma, Livin, cytochrome C