Abstract:

Abstract:Objective
To discuss the expressions  of the senile mice with cervical vertebral instability on Bcl-2 and Bax which related to neural apoptosis in hippocampal neurons,and to clarify the mechanism  of reducing of learning and memory abilities induced by cervical vertebral instability in the senile mice .Methods 20 ICR mice were selected and randomly  divided into two groups.The cervical vertebral instability model was established by lactic acid accumulation for long time;the changes of learning and memory abilities of the mice were observed with Watermaze test; the changes of morphology in hippocampal neurons were observed by HE straining;the apoptosis of hippocampal neurons was observed by Tunel staining.The expression levels of mRNA and protein of Bax and Bcl-2 were detected by RT-PCR and immunohistochemical staining .Results Compared with control group,the escape latency and wrong times of Watermaze test in  model group were increased(P<0.01) .The neuron in hippocampus in model group was found scatteredly and the  dead cells were increased obviously observed with HE staining compared with control group.The Tunel staining results showed that there were more buffy cells in model group(29.63%±1.63%) than control group（7.52%±0.99%）（P<0.01）.The expression of Bcl-2 was decreased and the Bax was increased in the brain tissue in model group compared with control group detected by RT-PCR and immunohistochemical staining.Conclusion The cervical vertebral instability could lead to Bcl-2 decreasing and Bax increasing,and it might be one of the main mechanisms to promote the apoptosis. 

Key words: cervical vertebral instability, hippocampus, Bcl-2, Bax, apoptosis