Abstract:

Abstract:Objective To investigate the differences in the structures,numbers and phenotypes of the dendritic cells (DCs) at different stages in colorectal cancer
(CRC) tissue, and to explore the effect of DCs in the occurrence and development of CRC.Methods 20 cases of CRC tissue were chosen as CRC
 group,other 20 cases of corresponding para-cancer tissue were chosen as control group.Immunohistochemical staining was used to detect the distributio
n,the structures,the number of positive cells at different stages;Western blotting was used to detect the expressions of CD1a and CD83.
Results The results of immunohistochemical staining showed that DCs scattered among glands or vessels in both groups;the CD1a+imature DCs (imDCs) were round and oval,dendritic processes were faintly visible in both groups,and the number of CD1a+imDCs in experimental group was significantly higher than that in control group (P<0.01).The CD83+ mature DCs (mDCs) were irregular,branched in both groups,and the number of CD83+mDCs in CRC  group was significantly lower than that in control group (P<0.01).The results of Western blotting method showed that the expression of CD1a protein was higher and the expression of CD83 protein was lower in CRC  group than  control group(P<0.01).Conclusion CD1a+imDCs may play important roles in promoting the occurrence and development of CRC;CD83+mDCs may inhibit the
 occurrence and development of CRC.

Key words: dendritic cells, colorectal neoplasms, immune response, immune tolerance