Abstract:

Abstract:Objective To observe the influence of rizatriptan on the behavior and pain-related cytokines in peripheral blood of the  migraine model rats,and to investigate the theraputic effect of rizatriptan on migraine.Methods A total of 24 rats were randomly divided into: control group,migraine  group,rizatriptan control group and rizatriptan treatment group.The rats in rizatriptan control and rizatriptan treatment groups were intragastrically perfused with rizatriptan,1 mg/kg per day (according to the adult daily dose),and the rats in  control and migraine  groups were perfused with normal saline, 1 mL per day.After 7 d,nitroglycerin was subcutaneously injected into the buttocks of the rats in rizatriptan treatment and migraine  groups to induce migraine.Normal saline  was injected into the rats in  control and rizatriptan control groups.At 60-90 min following nitroglycerin injection,the total number of behavioral symptoms was measured.The serum calcitonin gene-related peptide(CGRP),5-hydroxytryptamine(5-HT),interleukin-1β(IL-1β),and tumor necrosis factor-α(TNF-α) levels were determined using ELISA.Results Compared with migraine  group,the behavioral score of the rats in rizatriptan treatment group was significantly decreased (P<0.05). The serum CGRP level of the rats in migraine  group was significantly higher than that in  control group (P<0.05).Compared with  control group,the serum 5-HT level of the rats  in  and migraine  group was   decreased,but there was no significant differece(P>0.05);the serum 5-ＨＴ level in rizatriptan control group was significantly increased(P<0.05).The serum 5-HT level of the rats in rizatriptan treatment group was significantly increased compared with migraine  group (P<0.05).The serum IL-1β and TNF-α levels of the rats in varions groups had no significant differences (P>0.05).Conclusion Rizatriptan can relieve the  behavior symptoms in nitroglycerin-induced  migraine model rats,increase the  serum 5-HT level,improve the vasomotor disturbance,and relieve the angiectasis.

Key words: rizatriptan, calcitonin gene-related peptide, 5-hydroxytryptamine, interleukin-1&beta, tumor necrosis factor-&alpha