Abstract:

Objective To detect the percentages of CD4+,CD8+T lymphocytes in the breast invasive ductal carcinoma microenvironment, and to analyze their correlat
ion with breast carcinoma microvessel density (MVD).Methods The expressions of CD4,CD8 and CD34 were detected in 10 cases of adjacent normal breast tissue and 37 cases of breast invasive ductal carcinoma tissue by immunohistochemistry.The correlations between CD4,CD8,CD4/CD8 and MVD were analyzed by Spearman correlation analysis.Results The CD4 expression level (22.63%±11.53％) in breast invasive ductal carcinoma tissue was lower than that in adjacent tissue（34.09%±10.41％),and there was statistically significant difference (P<0.05).With the increasing of the histological grade of    breast cancer,,the expression level of CD4 was gradually decreased(P<0.05).The CD8 expression level (14.27%±9.03％) in breast invasive ductal carcinoma tissue was higher than that in adjacent tissue (10.51%±1.88％), but there was no statistically significant difference (P>0.05).With the increasing of the histological grade  of   breast cancer,the expression level of CD8 was gradually increased(P<0.01).The CD4/CD8 ratios in adjacent tissue group and breast invasi
ve ductal carcinoma tissue group were 3.24±1.02 and 2.16±2.17,and there was no statistically significant difference between them (P>0.05).
With the increasing of histological grade of breast invasive ductal carcinoma,the CD4/CD8 ratios were decreased (P<0.01).The MVD in breast invasive ductal carcinoma tissue was obviously higher than that in adjacent tissue,and it showed an increasing trend with the increasing of  the histological grade of breast invasive ductal carcinoma(P<0.05).The correlation analysis showed that the MVD  was negatively correlated with the CD4 expression（r=－1.000，P<
0.001） as well as CD4/CD8 ratio（r=－0.984，P<0.001）,and was positively correlated with the CD8  expression level
（r=0.984，P<0.001）.Conclusion The expression of CD4 is decreased in breast cancer tissue,the expression of CD8 is elevated,and the CD4/CD8 ratio is decreased,which suggests that T cells in the breast cancer microenvironment are immunosuppressed.
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Key words:  , breast neoplasms, T lymphocytes, tumor angiogenesis, CD4, CD8