Ndfip1对铁离子诱导的神经细胞凋亡的抑制作用及其神经保护作用机制

doi:10.13481/j.1671-587X.20210212

Abstract

Abstract: Objective

To observe the inhibitory effect of Nedd4 family interacting protein 1（Ndfip1）overexpression on the apoptosis of nerve cells under the conditions of iron overload， and to explore the protective mechanism of Ndfip1 in nerve cells.

Methods

The SH-SY5Y cells in experimental group were transfected with Ndfip1 plasmid， and the SH-SY5Y cells in control group were transfected with empty plasmid. MTT method was used to determine the survival rates of SH-SY5Y cells in two groups under the condition of iron overload. The levels of reactive oxygen species（ROS） and malondialdehyde （MDA） in the cells in two groups were detected by ROS and MDA detection kits. The expression levels of B-cell lymphoma-2 （Bcl-2）， Bcl-2-associated X protein （Bax） and cysteinyl aspartate specific proteinase-3 （caspase-3） proteins in the cells were detected by Western blotting method.

Results

Compared with control group， the survival rate of cells in experimental group was significantly increased （P<0.05）；compared with control group， the fluorescence of cells in experimental group was significantly weakened， indicating that the ROS level was reduced.Compared with control group， the level of MDA in experimental group was significantly reduced（P<0.05）；the expression level of Bcl-2 protein in the cells in experimental group was increased（P<0.05），and the expression levels of Bax protein and caspase-3 protein in the cells in experimental group were decreased（P<0.05）.

Conclusion

Ndfip1 could reduce the apoptosis induced by iron overload， improve the survival rate of nerve cells，and has protective effect on the nerve cells；its mechanism may be related to reducing the oxidative stress response and inhibiting the apoptosis pathway.

Key words: Nedd4 family interacting protein 1, iron, nerve cells, apoptosis, oxidative stress

CLC Number:

R329.3

Zhanqi ZHAO,Chengjin ZHANG,Juan TIAN. Inhibitory effect of Ndfip1 on ferrion-induced neuronal apoptosis and its neuroprotective mechanism[J].Journal of Jilin University(Medicine Edition), 2021, 47(2): 338-343.

Figures/Tables 2

References 25

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Inhibitory effect of Ndfip1 on ferrion-induced neuronal apoptosis and its neuroprotective mechanism

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