The human serous ovarian cancer cells were isolated and cultured from tumor tissue by type Ⅱ collagenase digestion and differential centrifugation method，and the third of generation serous ovarian cancer cells were used in subsequent experiments. The morphology of ovarian cancer cells was observed under inverted phase contrast microscope， and the cell growth curve was plotted and analyzed. The expression amounts of the human cancer antigen 125 （CA125）， paired box gene 8 （PAX8） and epididymis protein 4 （HE4） in the cells， and the expression amounts of embryonic stem cell homologous transcription factor （Nanog） and octamer binding transcription factor 4 （Oct-4） in the cancer stem cells were detected by immunofluorescence. The drug sensitivity test was divided into 5-fluorouracil （5-FU） groups （0， 0.625， 1.250， 2.500， 5.000 mg·L^－1）， paclitaxel （PTX） groups （0， 0.062 5， 0.125 0， 0.250 0， 0.500 0 mg·L^－1）， temozolomide （TMZ） groups （0， 0.125， 0.250， 0.500， 1.000 mg·L^－1）， carboplatin （CBP） groups （0， 0.625， 1.250， 2.500， 5.000 mg·L^－1）， adriamycin （DOX） groups （0， 0.125， 0.250， 0.500， 1.000 mg·L^－1） and melatonin （MLT） groups （0， 0.625， 1.250， 2.500， 5.000 mg·L^－1）；among them，0 mg·L^－1 groups were used as control group； CCK-8 method was used to evaluate the survival rates of the cells.

The primary serous ovarian cancer cells began to adhere to bottom of culture bottles 4 h after enzymatic digestion. The cells grew rapidly and had uniform morphology during continuous passages. The cells grew in a linearly phase during 2－7 d after inoculation， and grow and fuse into pieces after 8-10 days of culture. The results of immunofluorescence assay showed that CA125， PAX8，HE4， Nanog and Oct-4 were highly expressed in the cells. The drug sensitivity test showed that the ovarian cancer cells had high sensitivity to 5-FU，TMZ，CBP and MLT； compared with corresponding control groups，the survival rates of cells in different concentrations of 5-FU groups，TMZ groups and MLT groups and 0.0625 and 0.125 mg·L^－1 PTX groups were significantly decreased（P<0.05 or P<0.01）.

The culture system in vitro of human serous ovarian cancer cells is successfully established.The cells are sensitive to 5-FU， TMZ， CBP and MLT， which is of guiding significance for the selection of postoperative chemotherapy drugs in the patients with serous ovarian cancer.