CCK-8 method was used to determine the optimal concentration and time of MPTP with different concentrations （0.2， 0.4， 0.8， 1.6， and 3.2 mmol·L^－1）. CCK-8 method was used to determine the optimal concentration and time of lidocaine with different concentrations （0.001， 0.010， 0.100， 0.200， 0.400， 0.600， 0.800， and 1.000 g·L^－1）， and Western blotting method was used to further determine the optimal concentration of lidocaine. The PC12 cells were divided into control group， MPTP group（0.8 mmol·L^－1 MPTP for 24 h）， MPTP+ lidocaine group（given 0.8 mmol·L^－1 MPTP for 24 h， followed by 0.6 g·L^－1 lidocaine for 6 h）， MPTP+ lidocaine +DKK1 group（given 0.8 mmol·L^-1 MPTP for 24 h， followed by 0.6 g·L^－1 lidocaine and 100 μg·L^－1 DKK1 for 6 h）， and MPTP+DKK1 group（given 0.8 mmol·L^－1 MPTP for 24 h， followed by 100 μg·mL^－1 DKK1 for 6 h）. Flow cytometry was used to detect the levels of reactive oxygen species （ROS） in the PC12 cells in various groups. The expression levels of interleukin-1β（IL-1β）， interleukin-6（IL-6），and tumor necrosis factor-α（TNF-α） in supernatant of the PC12 cells in various groups were detected by ELISA assay；real-time fluorescence quantitative PCR （RT-qPCR） method was used to detect the expression levels of Wnt ligand protein 1 （Wnt1），β-catenin，and glycogen synthase kinase-3β（GSK-3β） mRNA in the PC12 cells in various groups.The expression levels of Wnt1， β-catenin， GSK-3β， phosphorylated GSK-3β （p-GSK-3β）， and cysteine aspartate protease 3 （caspase 3） proteins in the PC12 cells in various groups were detected by Western blotting method.

Compared with control group， the levels of ROS， IL-1β， IL-6 and TNF-α，and the expression levels of GSK-3β mRNA， and the expression levels of GSK-3β， p-GSK-3β， caspase 3 proteins in the PC12 cells in MPTP group were significantly increased（P<0.05）， and the expression levels of Wnt1， β-catenin mRNA and proteins were significantly decreased（P<0.05）. Compared with MPTP group， the levels of ROS， IL-1β， IL-6， and TNF-α and the expression levels of GSK-3β mRNA， and the expression levels of GSK-3β， p-GSK-3β， and caspase 3 proteins in the PC12 cells in MPTP+lidocaine group were significantly decreased（P<0.05）， however， the expression levels of Wnt1 and β-catenin mRNA and proteins were significantly increased（P<0.05）.

Lidocaine can significantly reduce the production of ROS and inhibit the release of pro-inflammatory cytokines in the PC 12 cells， and also significantly down-regulate the expression of apoptotic protein caspase 3， thus playing a protective role in the MPTP-induced PD model PC12 cells； its mechanism may be related to the Wnt /β-catenin signaling pathway.