Abstract:

Objective To discuss the effect of CD147 on the glycolysis of the prostate cancer （PCa） LNCaP cells， and to provide new molecular markers and targets for the clinical diagnosis and treatment of PCa. Methods The lentivirus infection system was used to establish the cells silencing CD147 as LNCaP/shCD147 group， and the negative control group （LNCaP/scramble group） was set up at the same time. The expression levels of CD147 mRNA in the LNCaP cells in two groups were detected by real-time fluorescent quantitative PCR （RT-qPCR）method； the concentrations of lactic acid （LA）， pyruvate （PA），and ATP in the LNCaP cells in two groups were detected by the kits； the activities of pyruvate kinase （PK），6-phosphofructokinase 1 （PFK1） and hexokinase （HK） were detected by the kits，and the expression levels of HK2， phosphofructokinase of muscle（PFKM），and pyruvate kinase M2（PKM2） proteins in the LNCaP cells in two groups were detected by Western blotting method. Results Compared with LNCaP/scramble group， the expression levels of CD147 mRNA and protein in the cells in LNCaP/shCD147 group were significantly decreased（P<0.01）；indicating that the PCa LNCaP cell model with silencing CD147 was successfully constructed.Compared with LNCaP/scramble group， the concentrations of LA， PA， and ATP in the cells in LNCaP/shCD147 group were significantly decreased （P<0.05 or P<0.01）. Compared with LNCaP/scramble group， the activities of PK， PFK1， and HK enzymes in the cells in LNCaP/shCD147 group were decreased （P<0.05 or P<0.01）.The Western blotting results showed that compared with LNCaP/scramble group， the expression level of HK2 protein in the cells in LNCaP/shCD147 group was decreased （P<0.01）. Conclusion Silencing CD147 inhibits the glycolysis of LNCaP cells by regulating the glycolytic metabolites of glycolysis， rate-limiting enzyme activity， and related protein expression levels.

Key words: CD147, Glycolysis, Prostate cancer, LNCaP cells, Hexo kinase 2