Abstract:

Objective To discuss the effect of polysaccharides from porphyra on the inflammatory factors secreted by macrophage RAW264.7 cells in the mice induced by lipopolysaccharide （LPS），and to clarify their immunomodulatory effect. Methods The RAW264.7 cells at logarithmic growth phase were divided into control group， LPS group， and LPS+polysaccharide from porphyra group. The viabilities of the cells in various groups were detected by CCK-8 method；the levels of interleukin-6 （IL-6），tumor necrosis factor-α （TNF-α）， monocyte chemotactic protein-1 （MCP-1）， macrophage inflammatory protein　（MIP）-1α，and MIP-1β in the cell culture supernatants in various groups were detected by enzyme-linked immunosorbent assay （ELISA） method. Results Compared with control group， the survival rate of the cells and the levels of IL-6， TNF-α， MCP-1， MIP-1α， and MIP-1β in the culture supernatant in LPS group and LPS+polysaccharide from porphyra group were significantly increased （P<0.01）. Compared with LPS group， the survival rate of the cells and the levels of IL-6， TNF-α， MCP-1， MIP-1α， and MIP-1β in the culture supernatant in LPS+polysaccharide from porphyra group were significantly decreased （P<0.01）. Conclusion polysaccharides from porphyra can inhibit the impact of the mouse macrophages on the secretion of inflammatory factors IL-6，TNF-α，MCP-1，MIP-1α，and MIP-1β，and have the potential immunomodulatory effect.

Key words: Polysaccharides from porphyra, Immune regulation, Macrophages, Inflammatory factor, Lipopolysaccharide