砾间接触氧化反应器中填料表面细菌多样性及其主要功能

砾间接触氧化反应器中填料表面细菌多样性及其主要功能

林山杉¹,金玉花¹,付丽丽¹,全成实²

1.东北师范大学城市与环境科学学院,长春 130024;2.吉林大学病理生物学教育部重点实验室,长春 130061

收稿日期:2006-11-20 修回日期:1900-01-01 出版日期:2007-03-26 发布日期:2007-03-26
通讯作者: 林山杉

Procaryotae Diversity on the Biofilm of Carrier and Their Function to Excess Sludge Demineralization in Gravel Contact Oxidation Reactor

LIN Shan-shan¹, JIN Yu-hua¹, FU Li-li¹, QUAN Cheng-shi²

1. School of City and Environmental Science, Northeast Normal University, Changchun 130024, China;2. The Key Laboratory of Pathobiology, Ministry of Education,Jilin University, Changchun 130061, China

Received:2006-11-20 Revised:1900-01-01 Online:2007-03-26 Published:2007-03-26
Contact: LIN Shan-shan

摘要/Abstract

摘要： 采用分子生物学手段，通过构建16S rDNA基因文库，对新型剩余污泥减量化处理系统—生物砾间接触氧化反应器 (GCOR) 中载体表面附着细菌多样性进行了系统发育分析，并讨论了多种细菌共存对剩余污泥减量化的贡献。结果表明，附着细菌可分为好氧呼吸菌群、兼性厌氧菌群、厌氧水解发酵菌群和生长缓慢菌群等4大类。其中，优势菌群分别是以兼具呼吸/发酵代谢方式的β变形菌、嗜水气单胞菌及以发酵为主要代谢方式的拟杆菌属。此外，好氧菌群中还发育有假单胞菌属、硝化螺菌属细菌和黄杆菌属细菌等。慢速生长菌群包括Caldimonas taiwanensis strain On 1和Ideonella sp.。填料表面生物膜微生态环境复杂，微生物多样性较高，好氧、厌氧菌群以及慢速生长菌群等多种细菌共同作用，为在降解污水有机物的同时，达到剩余污泥减量化做出巨大贡献。结果分析表明，细菌的主要功能可归纳为：能量解偶联、共代谢作用、生物溶胞作用和慢性生长种群的影响。

关键词: 生物砾间接触氧化反应器, 附着细菌群落结构, 16S rDNA基因文库, 系统发育分析

Abstract: Procaryotae diversity on the biofilm of carrier and their function to excess sludge demineralization in gravel contact oxidation reactor (GCOR) were studied by constructed 16SrRNA library, PCR-based molecular surveys of environmental 16S rDNA genes. The sequence analysis of 16S rDNA genes indicated that biofilm bacterial communities were composed of Proteobacteria, Bacteroidetes, Nitrobacteraceae and Unclassified bacteria group, respectivly. The most abundant sequences were those related to β-Proteobacteria group (20% clones from biolfilm). Next were those related to γ-Proteobacteria (20% clones from biofilm) and Bacteroides (about 13% clones from biofilm). A different kinds of bacteria contributed to excess sludge decrement were associated with uncoupled metabolism, diverse bacterial interactive behaviour, domination of slow growers and cryptic growth.

Key words: gravel contact oxidation reactor (GCOR), biofilm of carrier, 16S rDNA library, procaryotae diversity

中图分类号:

X143

林山杉,金玉花,付丽丽,全成实. 砾间接触氧化反应器中填料表面细菌多样性及其主要功能[J]. J4, 2007, 37(2): 361-366.

LIN Shan-shan, JIN Yu-hua, FU Li-li, QUAN Cheng-shi. Procaryotae Diversity on the Biofilm of Carrier and Their Function to Excess Sludge Demineralization in Gravel Contact Oxidation Reactor[J]. J4, 2007, 37(2): 361-366.

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