官厅水库生物固氮作用对水体富营养化的响应

J4 ›› 2011, Vol. 41 ›› Issue (4): 1179-1185.

官厅水库生物固氮作用对水体富营养化的响应

孙寓姣, 陈程, 丁爱中, 程莉蓉

北京师范大学水科学研究院|北京100875

收稿日期:2010-09-08 出版日期:2011-07-26 发布日期:2011-07-26
通讯作者: 丁爱中（1969-），男，安徽怀宁人，教授，博士生导师，主要从事水污染控制、水生态与环境修复研究 E-mail:ading@bnu.edu.cn
作者简介:孙寓姣(1975-)|女|黑龙江哈尔滨人|副教授|主要从事环境生物技术和污染环境微生物学方面的研究|E-mail:sunyujiao@bnu.edu.cn
基金资助:
国家自然科学基金项目(50708008)

Response of Biological Nitrogen Fixation to Eutrophic Water in Guanting Reservoir

SUN Yu-jiao, CHEN Cheng, DING Ai-zhong, CHENG Li-rong

College of Water Sciences, Beijing Normal University, Beijing100875, China

Received:2010-09-08 Online:2011-07-26 Published:2011-07-26

摘要/Abstract

摘要：

以官厅水库富营养化水体为研究对象，利用乙炔还原法测量水体生物固氮能力，结合变性梯度凝胶电泳（DGGE）技术对固氮生物nifH基因进行分析。实验结果表明，官厅水库水体存在生物固氮现象，固氮速率为0.172~9.35 nmol/(m³·d)，与水体氨氮、总氮、总磷呈显著相关性（P=0.998，r=0.000； P=0.986，r=0.000; P=0.968，r=0.002）。DGGE图谱显示：各点固氮细菌丰度差异较大，Shannon-Weaver指数为0.80~2.45，固氮细菌丰度和总氮、总磷存在较强相关性（P=0.737，r=0.094; P=0.787，r=0.063）。随着水体富营养水平的上升，固氮微生物多样性急剧增加，水体固氮能力也随之上升。自然固氮可能对原本富营养化的水体产生更大危害。

关键词: 官厅水库, 生物固氮, 富营养化, 聚合酶链式反应-变性梯度凝胶电泳

Abstract:

In Guanting reservoir, biological nitrogen fixation rate (NFR) was measured with acetylene reduction method, the diversity of nitrogen fixation bacteria (NFB) was revealed with nifH gene PCR-DGGE fingerprint. The results showed that biological nitrogen fixation exists in Guanting reservoir, the rates of nitrogen fixation ranged from 0.172 to 9.35 nmol/(m³·d). There was a positive correlation between NFR and NH⁺₄-N (P=0.998, r=0.000), TN (P=0.986, r=0.000), TP (P=0.968, r=0.002) concentrations, respectively. DGGE fingerprint indicated the (NFB) abundance differed between six sites and positive correlations were found between NFB abundance and TN (P=0.737, r=0.094), TP (P=0.787, r=0.063). Shannon-Weaver index varied in a range of 0.80-2.45. During the process of eutrophication in reservior, nitrogen fixation bacteria diversity increased with the nutrient status and thereby NFR enhanced. In addition, biological nitrogen fixation could aggravate eutrophication in Guanting reservoir.

Key words: Guanting reservoir, biological nitrogen fixation, eutrophication, PCR-DGGE

中图分类号:

X524

孙寓姣, 陈程, 丁爱中, 程莉蓉. 官厅水库生物固氮作用对水体富营养化的响应[J]. J4, 2011, 41(4): 1179-1185.

SUN Yu-jiao, CHEN Cheng, DING Ai-zhong, CHENG Li-rong. Response of Biological Nitrogen Fixation to Eutrophic Water in Guanting Reservoir[J]. J4, 2011, 41(4): 1179-1185.

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