蛋清生物活性肽组分的优选

吉林大学学报(工学版) ›› 2010, Vol. 40 ›› Issue (增刊): 389-0394.

蛋清生物活性肽组分的优选

刘静波¹,王莹²,郭洋¹,许海丽¹,林松毅¹,殷涌光²

1.吉林大学军需科技学院营养与功能食品研究室,长春 130062;2.吉林大学生物与农业工程学院,长春 130022

收稿日期:2010-01-21 出版日期:2010-09-01 发布日期:2010-09-01
通讯作者: 林松毅（1970-）,女,副教授，硕士生导师.研究方向:功能食品研究与开发.E-mail:linsongyi730@163.com E-mail:linsongyi730@163.com
作者简介:刘静波（1962-）,女,教授,硕士生导师.研究方向:营养与功能食品.E-mail:ljb168@sohu.com
基金资助:
“863”国家高技术研究发展计划项目（2007AA10Z329）；吉林大学科学前沿与交叉学科创新项目(200810029)

Optimization of bioactive peptides derived from egg white protein

LIU Jing-bo¹,WANG Ying²,GUO Yang¹,XU Hai-li¹,LIN Song-yi¹,YIN Yong-guang²

1.Laboratory of Nutrition and Functional Food|College of Quartermaster Technology|Jilin University| Changchun 130062,China；2.College of Biological and Agricultural Engineering,Jilin University,Changchun 130022,China

Received:2010-01-21 Online:2010-09-01 Published:2010-09-01

摘要/Abstract

摘要：

以优选出不影响食物摄取量和体重变化的蛋清生物活性肽组分为研究目的，以受试小鼠的体重、平均日摄食量为衡量指标，对比分析了蛋清生物活性肽在受试30 d和90 d内的差异。研究发现：无论是经碱性蛋白酶一次酶解制得的蛋清蛋白酶解物，还是经碱性蛋白酶和风味蛋白酶双酶酶解制得的蛋清高F值寡肽，在受试30 d和90 d的对比时间内，对体重没有显著影响（p>0.05）；在灌胃30 d蛋清生物活性肽及阳性对照物（大豆提能肽）的对比分析中发现，受试物可不同程度降低对受试30 d小鼠的平均日摄食量，从30 d时间上考察没有显著性的影响（p>0.05）；在灌胃30 d不同浓度蛋清生物活性肽的研究中发现，除灌胃30 d、4.5 mg/（g·bw·d）蛋清高F值寡肽外，其余实验组未在受试30 d的时间内表现出影响平均日摄食量的作用（p>0.05）；在灌胃90 d不同蛋清生物活性肽对比分析中发现，除4.5 mg/（g·bw·d）一次酶解4 h得到的蛋清蛋白酶解物外，其余受试物均可以显著降低小鼠的平均日摄食量（p<0.05或p<0.01）。

关键词: 蛋清生物活性肽, 食物摄取量, 体重变化, 差异显著性

Abstract:

In this paper, the body weight and average daily food intake of mice which were reared for 30 d or 90 d were determined in order to optimizate different compound of the bioactive peptides derived from egg white protein which had no effect on body weight changing condition and food intake. The results showed that: no matter the egg white hydrolysates which only hydrolyzed by Alaclase or the high F ratio oligopeptide under through both Alaclase and flavorenzyme, they had no significantly effect on the body weight of mice reared for 30 d and 90 d compared to the blank group (p>0.05). Egg white hydrolysates and the positive control (Tineng soybean peptide)could reduce the average mouse food intake in different degrees, but they had no significantly effect compared to the blank group (p>0.05) in 30 d. Except for 4.5 mg/(g·bw·d), different concentration of high F ratio oligopeptide also had no significantly effect compared to the blank group (p>0.05) at 30 d. During considering the effect of different bioactive peptide from egg white protein on food intake of mice which were reared for 90d, we found that except for the EWPE4, bioactive peptide from egg white protein could significantly decrease the average daily feed intake (p<0.05 or p<0.01).

Key words: bioactive peptide derived from egg whiter protein, food intake, body weight changing condition, significance

中图分类号:

TS201

刘静波, 王莹, 郭洋, 许海丽, 林松毅, 殷涌光. 蛋清生物活性肽组分的优选[J]. 吉林大学学报(工学版), 2010, 40(增刊): 389-0394.

LIU Jing-Bo, WANG Ying, GUO Yang, XU Hai-Li, LIN Song-Yi, YIN Yong-Guang. Optimization of bioactive peptides derived from egg white protein[J]. 吉林大学学报(工学版), 2010, 40(增刊): 389-0394.

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