响应曲面法优化羊肚菌富硒深层发酵条件

吉林大学学报(工学版) ›› 2013, Vol. 43 ›› Issue (增刊1): 557-563.

响应曲面法优化羊肚菌富硒深层发酵条件

丁健峰, 孙永海, 付天宇, 谢高鹏

吉林大学生物与农业工程学院,长春130022

收稿日期:2011-10-18 发布日期:2013-06-01
通讯作者: 孙永海(1956-),男,教授,博士生导师.研究方向:功能食品.E-mail:sunyh@jlu.edu.cn E-mail:sunyh@jlu.edu.cn
作者简介:丁健峰(1986-),男,硕士研究生.研究方向:功能食品.E-mail:samdjf@163.com
基金资助:
吉林省科技发展计划重点项目(20110247);国家自然科学基金项目(3127186).

Response surface methodology optimization of Morchella mycelium selenium enriched fermentation

DING Jian-feng, SUN Yong-hai, Fu Tian-yu, Xie Gao-peng

College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, China

Received:2011-10-18 Published:2013-06-01

摘要/Abstract

摘要：

以亚硒酸钠为无机硒源,对羊肚菌菌丝体的富硒深层发酵条件进行了优化研究。选择培养温度、发酵时间和硒浓度作为优化因素,研究各因素的不同水平对富硒羊肚菌菌丝生物量和硒含量的影响。通过单因素试验和响应曲面法得出了羊肚菌菌丝体富硒深层发酵的最佳条件条件:发酵时间5.22d、培养温度25.73℃、硒质量浓度86.67μg/ml;预测菌丝生物量的达到10.512g/L、硒含量达到18.8μg/ml,实际菌丝生物量的达到10.339g/L、硒含量达到18.6μg/ml。研究结果表明,响应曲面法可对羊肚菌富硒深层发酵条件进行优化。

关键词: 食品科学技术, 羊肚菌, 深层发酵, 富硒, 响应曲面法

Abstract:

Sodium selenite was used as inorganic selenium source in Morchella mycelium enriched selenium submerged fermentation. The optimal fermentation parameters were researched. Selected culture temperature, fermentation time and selenium concentration as the optimized factors. Influence of levels of the factors on enriched selenium Morchella mycelium biomass and the selenium content were studied. Through the single factor tests and response surface method the optimal processing conditions of Morchella mycelium enriched selenium submerged fermentation was concluded. They are fermentation time 5.22d, culture temperature 25.73 ℃, selenium concentration 86.67 μg/ml; Prediction of mycelium biomass is 10.512 g/L, selenium content 18.8 μg/ml. Actual mycelium biomass is up to 10.339 g/L, selenium content 18.6 μg/ml. The results show that the response surface method is a most useful tool in Morchella selenium-rich submerged fermentation conditions optimization.

Key words: food science and technology, morchella, submerged fermentation, Se-accumulating, response surface methodology

中图分类号:

S567.39

丁健峰, 孙永海, 付天宇, 谢高鹏. 响应曲面法优化羊肚菌富硒深层发酵条件[J]. 吉林大学学报(工学版), 2013, 43(增刊1): 557-563.

DING Jian-feng, SUN Yong-hai, Fu Tian-yu, Xie Gao-peng. Response surface methodology optimization of Morchella mycelium selenium enriched fermentation[J]. 吉林大学学报(工学版), 2013, 43(增刊1): 557-563.

参考文献

[1] Mahan D C. Effects of seleniumr enriched yeast and sodium selenite on tissue selenium retentin and serum glutathione peroxidase activity in grower and finisher swine[J].J Anim Sci,1999, 74:2967-2974.

[2] John Weldon Finley.The absorption and tissue distribution of selenium from high-selenium broccoli are different from selenium sodium selenite,sodium selenate and selenomethionine as determined in selenium-deficient rats[J].J Agic Food Chem ,1998,46:3702-3707.

[3] 姚莉,段玉峰.微量元素硒与人体健康[J].中国食物与营养,2004,7:50-52. Yao Li,Duan Yu-feng.Selenium and health[J].Food and Nutrition In China,2004,7:50-52.

[4] 常海波,孙凤春,楚玉彪.微量元素硒与人体健康的研究[J].世界元素医学,2006,13(2):22- 23. Chang Hai-bo,Sun Feng-chun,Chu Yu-biao. Study on the relationship between Se and human health[J]. World Elemental Medlcine,2006,13(2):22- 23.

[5] 宫丽,马光.硒元素与健康[J].环境科学与管理,2007, 32(9): 32-35. Gong Li,Ma Guang.The selenium elemental and health[J].Environmental Science and Management, 2007, 32(9): 32-35.

[6] Rayman M.The importance of selenium to human health[J].Lancet,2000,356:233-241.

[7] 暴增海,马桂珍,徐晓红.微量元素对食用菌产量和品质的影响[J].世界农业,2002,7:38-40. Bao Zeng-hai,Ma Gui-zhen,Xu Xiao-hong.Effects of microelement on the yield and quality of edible fungi[J].World Agriculture,2002,7:38-40.

[8] 魏芸,张天佑,张姝,等.羊肚菌多糖的分离纯化及组成结构分析[J].植物资源与环境学报, 2000,4(04):36-37. Wei Yun,Zhang Tian-you,Zhang Shu, et al. Isolation, purification and characterization of polysaccharides from Morchella essulenta L.[J].Journal of plant resources and Environment,2000,4(04):36-37.

[9] 张月,钟鸣,林景卫,等.紫外分光光度法测定蛹虫草菌丝体中硒的含量[J].微量元素与健康研究,2010,5:21-23. Zhang Yue,Zhong Ming,Lin Jing-Wei,et al.Ultraviolet spectrophotometry determine selenium content in cordyceps militaris mycelium[J].Studies of Trace Elements and Health, 2010,5:21-23.

[10] 周跃花,杨菊香,张娜.紫外分光光度法测定紫阳富硒茶中的硒[J].西北农业学报,2009,18(4);229-232. Zhou Yue-hua,Yang Ju-xiang,Zhang Na. Determination of selenium of enriched tea in ziyang by UV spectrophotometry[J].Acta Agriculturae Boreali-Occidentalis Sinica, 2009,18(4);229-232.

[11] 张润楚,邓海涛,兰燕,等.试验设计与分析及参数优化[M].北京:中国统计出版社,2003:346- 350.

相关文章 10

[1]	陈莉, 孙永海, 付天宇, 丁健峰. 羊肚菌胞外多糖快速估测方法[J]. 吉林大学学报(工学版), 2014, 44(2): 567-572.
[2]	刘静波, 安晓宁, 梁春玮, 王作昭, 王二雷, 张燕, 姜轶群. 微波提取鸡蛋黄中类胡萝卜素工艺的优化[J]. 吉林大学学报(工学版), 2013, 43(06): 1712-1718.
[3]	徐彩娜, 殷涌光, 王二雷, 刘静波. 卵黄高磷蛋白及其磷酸肽稳定性和抗氧化性[J]. 吉林大学学报(工学版), 2013, 43(02): 544-549.
[4]	刘晶晶, 孙永海, 丁健峰, 孙钟雷. 玉米汁辨识中的传感器阵列优化[J]. 吉林大学学报(工学版), 2013, 43(02): 538-543.
[5]	刘静波, 王菲, 张燕, 王二雷, 王作昭, 姜轶群. 蛋清源凝血酶抑制肽的纯化[J]. 吉林大学学报(工学版), 2012, 42(增刊1): 466-469.
[6]	赵谋明, 孔静, 刘丽娅, 赵强忠. 黄原胶/CMC复合稳定剂对酪蛋白乳液稳定性的影响[J]. , 2012, 42(05): 1343-1348.
[7]	刘静波, 马爽, 林松毅, 刘博群, 杨军, 王二雷. 速溶蛋黄粉喷雾干燥工艺优化及其特性[J]. , 2012, 42(05): 1336-1342.
[8]	刘静波, 王翠娜, 刘军, 路爽, 张城, 蔡玥. 玉米须急性毒性及致突变性[J]. 吉林大学学报(工学版), 2011, 41(增刊2): 359-363.
[9]	刘静波, 王翠娜, 刘军, 张城, 路爽, 蔡玥. 玉米须对Wistar大鼠神经行为的毒性作用[J]. 吉林大学学报(工学版), 2011, 41(增刊2): 354-358.
[10]	庄红, 崔真真, 刘静波. 长白山矿泉水的功能特性[J]. 吉林大学学报(工学版), 2010, 40(增刊): 400-0403.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed