吉林大学学报(工学版) ›› 2013, Vol. 43 ›› Issue (增刊1): 557-563.

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Response surface methodology optimization of Morchella mycelium selenium enriched fermentation

DING Jian-feng, SUN Yong-hai, Fu Tian-yu, Xie Gao-peng   

  1. College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, China
  • Received:2011-10-18 Published:2013-06-01

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Abstract:

Sodium selenite was used as inorganic selenium source in Morchella mycelium enriched selenium submerged fermentation. The optimal fermentation parameters were researched. Selected culture temperature, fermentation time and selenium concentration as the optimized factors. Influence of levels of the factors on enriched selenium Morchella mycelium biomass and the selenium content were studied. Through the single factor tests and response surface method the optimal processing conditions of Morchella mycelium enriched selenium submerged fermentation was concluded. They are fermentation time 5.22d, culture temperature 25.73 ℃, selenium concentration 86.67 μg/ml; Prediction of mycelium biomass is 10.512 g/L, selenium content 18.8 μg/ml. Actual mycelium biomass is up to 10.339 g/L, selenium content 18.6 μg/ml. The results show that the response surface method is a most useful tool in Morchella selenium-rich submerged fermentation conditions optimization.

Key words: food science and technology, morchella, submerged fermentation, Se-accumulating, response surface methodology

CLC Number: 

  • S567.39

[1] Mahan D C. Effects of seleniumr enriched yeast and sodium selenite on tissue selenium retentin and serum glutathione peroxidase activity in grower and finisher swine[J].J Anim Sci,1999, 74:2967-2974.

[2] John Weldon Finley.The absorption and tissue distribution of selenium from high-selenium broccoli are different from selenium sodium selenite,sodium selenate and selenomethionine as determined in selenium-deficient rats[J].J Agic Food Chem ,1998,46:3702-3707.

[3] 姚莉,段玉峰.微量元素硒与人体健康[J].中国食物与营养,2004,7:50-52. Yao Li,Duan Yu-feng.Selenium and health[J].Food and Nutrition In China,2004,7:50-52.

[4] 常海波,孙凤春,楚玉彪.微量元素硒与人体健康的研究[J].世界元素医学,2006,13(2):22- 23. Chang Hai-bo,Sun Feng-chun,Chu Yu-biao. Study on the relationship between Se and human health[J]. World Elemental Medlcine,2006,13(2):22- 23.

[5] 宫丽,马光.硒元素与健康[J].环境科学与管理,2007, 32(9): 32-35. Gong Li,Ma Guang.The selenium elemental and health[J].Environmental Science and Management, 2007, 32(9): 32-35.

[6] Rayman M.The importance of selenium to human health[J].Lancet,2000,356:233-241.

[7] 暴增海,马桂珍,徐晓红.微量元素对食用菌产量和品质的影响[J].世界农业,2002,7:38-40. Bao Zeng-hai,Ma Gui-zhen,Xu Xiao-hong.Effects of microelement on the yield and quality of edible fungi[J].World Agriculture,2002,7:38-40.

[8] 魏芸,张天佑,张姝,等.羊肚菌多糖的分离纯化及组成结构分析[J].植物资源与环境学报, 2000,4(04):36-37. Wei Yun,Zhang Tian-you,Zhang Shu, et al. Isolation, purification and characterization of polysaccharides from Morchella essulenta L.[J].Journal of plant resources and Environment,2000,4(04):36-37.

[9] 张月,钟鸣,林景卫,等.紫外分光光度法测定蛹虫草菌丝体中硒的含量[J].微量元素与健康研究,2010,5:21-23. Zhang Yue,Zhong Ming,Lin Jing-Wei,et al.Ultraviolet spectrophotometry determine selenium content in cordyceps militaris mycelium[J].Studies of Trace Elements and Health, 2010,5:21-23.

[10] 周跃花,杨菊香,张娜.紫外分光光度法测定紫阳富硒茶中的硒[J].西北农业学报,2009,18(4);229-232. Zhou Yue-hua,Yang Ju-xiang,Zhang Na. Determination of selenium of enriched tea in ziyang by UV spectrophotometry[J].Acta Agriculturae Boreali-Occidentalis Sinica, 2009,18(4);229-232.

[11] 张润楚,邓海涛,兰燕,等.试验设计与分析及参数优化[M].北京:中国统计出版社,2003:346- 350.
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