Abstract:

In order to develop alkylpolyglycoside(APG)which is a highpurity and environment friendly biosurfactant,the processparameters of APG synthesis catalyzed by ACA microcapsuleim mobilized enzyme were optimized through single factor experimentation and twice or thogonalrotating combination igntest,andthe model of glucose conversion rate was studied.The results show that factors which have influence onglucose conversion rate are:reaction time,amount of adding water,reaction temperature and amount of alcohol,what’s more,the formeris better than the latter;reaction temperature is more interactive with a mount of alcohol,amount of adding water,reaction time,a mount of alcohol and amount of adding water is the same;the best synthesis process parameters are as follows:reaction temperature at 45 ℃,amount of dodecanol of 8 mL,immobilized enzyme of 5 g,amount of glucose of 1 g,without adding water,reaction time of 5days and the maximum of glucose conversionrate is97.9%.

Key words: food industry, ACA microcapsule immobilized enzyme, β-glucosidase, alkylpolygly coside, process optimization, mathe matical model