siRNA序列特征的RNA干扰效率预测的新方法

J4 ›› 2011, Vol. 29 ›› Issue (5): 436-443.

siRNA序列特征的RNA干扰效率预测的新方法

徐宝林^a,于大川^b,高冬^a,张浩^a,刘元宁^a

吉林大学 a.计算机科学与技术学院|长春 130012；b.第一医院疼痛科, 长春 130021

出版日期:2011-09-24 发布日期:2011-11-29
作者简介:徐宝林(1988—)|男|河南光山人|吉林大学硕士研究生|主要从事siRNA设计研究|（Tel）86-13756187203（E-mail）baolinx@gmail.com;刘元宁(1962—),男|辽宁抚顺人,吉林大学教授|博士生导师|主要从事生物信息学研究|（Tel）86-13904336786（E-mail）lyn@jlu.edu.cn
基金资助:
国家自然科学基金资助项目(60971089)

Method for Prediction of RNA Interference Efficiency Based on Sirna Sequence Characteristics

XU Bao-lin^a,YU Da-chuan^b,GAO Dong^a,ZHANG Hao^a,LIU Yuan-ning^a

a. College of Computer Science and Technology, Jilin University,Changchun 130012,China;b. Department of Pain,The First Hospital of Jilin University,Changchun 130021,China

Online:2011-09-24 Published:2011-11-29

摘要/Abstract

摘要：

为了快速、准确预测siRNA(Small interfering Ribonucleic Acid)的干扰效率，通过对2431条siRNA的统计学分析找到了一个可靠的预测新方法。通过统计学方法从众多特征中筛选出了19个与siRNA抑制效率相关的序列特征，并用这些特征给出了siRNA抑制效率水平的聚类方法，准确率达到81.1%。该方法将对高效的siRNA设计起到指导意义。

关键词: RNA干扰, 干扰效率预测, 序列特征, 统计学分析

Abstract:

To predict the siRNA(Small interfering Ribonucleic Acid) efficiency accurately, by the statistical analysis of 2 431 siRNA,we found a reliable new method of forecasting.We selected 19 sequence characteristics related to siRNA efficiency with statistical method and made out a clustering method for siRNA inhibiting efficiency level with these characteristics,the correct rate is up to 81.1%. This method provides orientation for high efficient siRNA design.

Key words: ribonucleic acid interference(RNAi), prediction of interference efficiency, sequence characteristic, statistical analysis

中图分类号:

TP399

徐宝林, 于大川, 高冬, 张浩, 刘元宁. siRNA序列特征的RNA干扰效率预测的新方法[J]. J4, 2011, 29(5): 436-443.

XU Bao-Lin, XU Da-Chuan, GAO Dong, ZHANG Gao, LIU Yuan-Ning. Method for Prediction of RNA Interference Efficiency Based on Sirna Sequence Characteristics[J]. J4, 2011, 29(5): 436-443.

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