关键词: Tca8113细胞, 包含WW域的氧化还原酶基因, 口腔鳞状细胞癌, 基因芯片

Abstract: Objective: To analyze the changes of gene profile of over-expression WWOX gene in the human oral squamous cell carcinoma(OSCC) Tca8113 cells, and to explore the tumor inhibition mechanism of WWOX gene.Methods: The Tca8113 cells were divided into WWOX over-expression group and control group. In WWOX over-expression group, the lentiviral plasmid carrying the full-length cDNA fragment of WWOX gene was infected into the Tca8113 cells, while in control group, the lentiviral plasmid carrying the empty pGV287-LV vector was infected into the Tca8113 cells. The quantitative real time PCR (qRT-PCR)and Western blotting methods were used to detect the expressions of WWOX mRNA and protein. The gene chip technique was used to screen the differientially expressed genes and biological analysis was performed. The quantitative PCR was used to detect the relative mRNA expression levels of differientially expressed genes involving in the mitogen-activated protein kinase(MAPK) signaling pathway.Results: After infection, the relative mRNA expression level of WWOX gene in the Tca8113 cells in WWOX over-expression group was higher than that in control group(P<0.05), and the expression of WWOX protein in the Tca8113 cells in WWOX over-expression group was higher. A total of 347 differientially expressed genes with a 1.5-fold-change(FC) were screened by gene chip, in which 171 genes showed up-regulated expression, and 176 genes showed down-regulated expression. The Bioinformation analysis results showed that these different genes distributed in several pathways, which were related to cancer, p53, MAPK, and interleukin-2(IL-2) pathways and so on. Compared with control group,the relative expression levels of MAP2K,NR4A1,DUSP5,and DUSP6 mRNA in MAPK signaling pathway in WWOX over-expression group were increased(P<0.05), and the relative expression level of fibroblast growth factor receptor 2(FGFR2) mRNA was decresed(P<0.05).Conclusion: The over-expression of WWOX gene in the Tca8113 cells can induce the changes of gene profile of Tca8113 cells. The tumor inhibition mechanism of WWOX gene may be related to regulating the expressions of some genes involving in MAPK signaling pathway.

Key words: Tca8113 cells, WW domain-containing oxidoreductase gene, oral squamous cell carcinoma, gene chip