牙髓干细胞增殖、分化和迁移的研究进展

doi:10.13481/j.1671-587x.20180540

摘要/Abstract

摘要： 牙髓干细胞（DPSCs）是一种与骨髓间充质干细胞（BMSCs）有着极其相似的免疫表型及形成矿化结节能力的细胞，细胞形态呈梭形，可自我更新和多向分化，有着较强的克隆能力。随着DPSCs研究方向的多元化、研究方法的多样化以及研究层次的深入化，目前DPSCs已成为国内外研究热点，并成为组织工程中重要的种子细胞之一，提示DPSCs未来具有应用于临床治疗的潜能，以期恢复机体受损组织及器官的形态和功能。近些年来，研究者发现DPSCs的增殖受到多种效应的调控，其迁移及归巢特性受到多种细胞因子及趋化因子的调节，并发现其不仅可以形成牙本质等牙体硬组织，在特定诱导环境下可以向多种组织器官定向分化。本文系统地回顾了近年来国内外相关文献，重点阐述了有关DPSCs增殖、迁移和分化等的最新研究成果，以期为DPSCs在转化医学及临床应用方面的研究提供新的思路和策略。

关键词: 牙髓干细胞, 细胞增殖, 细胞迁移, 细胞分化

中图分类号:

R780.2

刘定坤, 杨楠, 金巨楼, 孙梦洁, 王倩, 吴佳, 刘志辉. 牙髓干细胞增殖、分化和迁移的研究进展[J]. 吉林大学学报(医学版), 2018, 44(05): 1100-1104.

参考文献

[1] Gronthos S, Mankani M, Brahim J, et al. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo[J]. Proc Natl Acad Sci USA, 2000, 97(25):13625-13630.
[2] Li Y, He L, Pan S, et al. Three-dimensional simulated microgravity culture improves the proliferation and odontogenic differentiation of dental pulp stem cell in PLGA scaffolds implanted in mice[J]. Mol Med Rep, 2017, 15(2):873-878.
[3] Bhuptani RS, Patravale VB. Porous microscaffolds for 3D culture of dental pulp mesenchymal stem cells[J]. Int J Pharm, 2016, 515(1/2):555-564.
[4] Lew W Z, Huang Y C, Huang K Y, et al. Static magnetic fields enhance dental pulp stem cell proliferation by activating the p38 MAPK pathway as its putative mechanism[J]. J Tissue Eng Regen Med, 2016, 12(1):19-29.
[5] Kanafi MM, Ramesh A, Gupta PK, et al. Influence of Hypoxia, High Glucose, and Low Serum on the Growth Kinetics of Mesenchymal Stem Cells from Deciduous and Permanent Teeth[J]. Cells Tissues Organs(Print), 2013, 198(3):198-208.
[6] 万千千,侯立鹏,韩冰,等.碱性成纤维细胞生长因子对3D培养条件下牙髓干细胞成牙分化的研究[J].牙体牙髓牙周病学杂志,2018,28(3):125-130.
[7] 车静怡,李艳萍,潘爽,等.黄瓜籽正丁醇提取物对人牙髓干细胞增殖及成牙向分化的影响[J].口腔医学研究,2018,34(4):380-383.
[8] Soares DG, Rosseto HL, Basso FG, et al. Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cells[J]. Braz Oral Res,2016,30(1):e54.
[9] Khojasteh A, Motamedian SR, Rad MR, et al. Polymeric vs hydroxyapatite-based scaffolds on dental pulp stem cell proliferation and differentiation[J]. World J Stem Cells, 2015, 7(10):1215-1221.
[10] Ling L, Zhao YM, GELH. Impact of different degree pulpitis on cell proliferation and osteoblastic differentiation of dental pulp stem cell in Beagle immature premolars[J]. J Peking Univ HealthSci, 2016, 48(5):878-883.
[11] Wen Q, Zhao YM, Wang YY, et al. Effects of stromal cell-derived factor-1 on proliferation, migration, and odontoblastic differentiation of human dental pulp stem cells[J]. J Peking Univ Health Sci, 2016, 48(1):23-29.
[12] Li M, Sun X, Ma L, et al. SDF-1/CXCR4 axis induces human dental pulp stem cell migration through FAK/PI3K/Akt and GSK3β/β-catenin pathways[J]. Sci Rep,2017, 7:40161.
[13] Yang JW, Zhang YF, Wan CY, et al. Autophagy in SDF-1α-mediated DPSC migration and pulp regeneration[J]. Biomaterials, 2015, 44:11-23.
[14] Zeng Q, Nguyen S, Zhang H, et al. Release of Growth Factors into Root Canal by Irrigations in Regenerative Endodontics[J]. J Endod, 2016, 42(12):1760-1766.
[15] 刘宏胜, 白小文, 杨媛,等. 人类年轻恒牙牙髓干细胞体外多向分化的能力[J]. 北京大学学报:医学版, 2007, 39(1):41-45.
[16] 何飞, 谭颖徽, 张纲. 人牙髓干细胞的体外培养和鉴定[J]. 华西口腔医学杂志, 2005, 23(1):75-78.
[17] Laino G, Carinci F, Graziano A, et al. In vitro bone production using stem cells derived from human dental pulp[J]. J Craniofac Surg, 2006, 17(3):511-515.
[18] Almushayt A, Narayanan K, Zaki AE, et al. Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts[J]. Gene Ther, 2006, 13(7):611-620.
[19] Zhang W, Walboomers XF, Shi S, et al. Multilineage differentiation potential of stem cells derived from human dental pulp after cryopreservation[J]. Tissue Eng, 2006, 12(10):2813-2823.
[20] Li Y, Zhao S, Nan X, et al. Repair of human periodontal bone defects by autologous grafting stem cells derived from inflammatory dental pulp tissues[J]. Stem Cell Res Ther, 2016, 7(1):141.
[21] Hozhabri NS, Benson MD, Vu MD, et al. Decreasing NF-κB expression enhances odontoblastic differentiation and collagen expression in dental pulp stem cells exposed to inflammatory cytokines[J]. PLoS One, 2015, 10(1):e0113334.
[22] Yi Q, Liu O, Yan F, et al. Analysis of senescence-related differentiation potentials and gene expression profiles in human dental pulp stem cells[J]. Cells Tissues Organs (Print), 2017, 203(1):1-11.
[23] Wu Y, Huang F, Zhou X, et al. Hypoxic preconditioning enhances dental pulp stem cell therapy for infection-caused bone destruction[J]. Tissue Eng Part A, 2016, 22(19/20):1191-1203.
[24] Rosa V, Xie H, Dubey N, et al. Graphene oxide-based substrate:physical and surface characterization, cytocompatibility and differentiation potential of dental pulp stem cells[J]. Dent Mater, 2016, 32(8):1019-1025.
[25] Yu W, Zhang Y, Jiang C, et al. Orthodontic treatment mediates dental pulp microenvironment via IL17A[J]. Arch Oral Biol, 2016, 66:22-29.
[26] Jia W, Zhao Y, Yang J, et al. Simvastatin Promotes Dental Pulp Stem Cell-induced Coronal Pulp Regeneration in Pulpotomized Teeth[J]. J Endod, 2016, 42(7):1049-1054.
[27] Lauritano D, Avantaggiato A, Candotto V, et al. Insulin activity on dental pulp stem cell differentiation:an in vitro study[J]. J Biol Regul Homeost Agents, 2015, 29(3 Suppl 1):48-53.
[28] Wang L, Cheng L, Wang H, et al. Glycometabolic reprogramming associated with the initiation of human dental pulp stem cell differentiation[J]. Cell Biol Int, 2016, 40(3):308-317.
[29] Li D, Deng T, Li H, et al. MiR-143 and miR-135 inhibitors treatment induces skeletal myogenic differentiation of human adult dental pulp stem cells[J]. Arch Oral Biol, 2015, 60(11):1613-1617.
[30] Kim D, Kim J, Hyun H, et al. A nanoscale ridge/groove pattern arrayed surface enhances adipogenic differentiation of human supernumerary tooth-derived dental pulp stem cells in vitro[J]. Arch Oral Biol, 2014, 59(8):765-774.
[31] Chen YK, Huang AH, Chan AW, et al. Human dental pulp stem cells derived from cryopreserved dental pulp tissues of vital extracted teeth with disease demonstrate hepatic-like differentiation[J]. J Tissue Eng Regen Med, 2016, 10(6):475-485.
[32] Westin CB, Trinca RB, Zuliani C, et al. Differentiation of dental pulp stem cells into chondrocytes upon culture on porous chitosan-xanthan scaffolds in the presence of kartogenin[J]. Mater Sci Eng C Mater Biol Appl, 2017, 80:594-602.
[33] 吴中明, 木合塔尔·霍加, 买布拜木·买买提依明,等. 牙髓干细胞在实验兔牙槽骨缺损修复中的作用研究[J]. 中华细胞与干细胞杂志:电子版, 2015, 5(3):170-175.
[34] El-Backly RM, Massoud AG, El-Badry AM, et al. Regeneration of dentine/pulp-like tissue using a dental pulp stem cell/poly(lactic-co-glycolic) acid scaffold construct in New Zealand white rabbits[J]. Aust Endod J, 2008, 34(2):52-67.
[35] 胡资兵, 曾荣, 魏波,等. 牙髓干细胞移植治疗大鼠脊髓损伤的实验研究[J]. 中国脊柱脊髓杂志, 2014, 24(9):839-846.
[36] 王伟, 陈超, 刘延明,等. 应用小动物活体成像追踪观察EGFP标记的间充质干细胞[J]. 中国细胞生物学学报, 2016,38(2):185-192.
[37] 仵韩,木合塔尔·霍加,古扎丽努尔·阿巴拜克力.转化生长因子-β3对牙髓干细胞成骨向分化的作用[J].临床口腔医学杂志,2017,33(10):596-599.
[38] Li JH, Liu DY, Zhang FM,et al. Human dental pulp stem cell is a promising autologous seed cell for bone tissue engineering[J]. Chin Med J, 2011, 124(23):4022-4028.