Abstract:

Rana chensinensis Changbaishanensis oviduct glycoprotein was separated and identified in the test. Saturated ammonium sulfate was uesd to pre
cipitate Rana c Changbaishanensis oviduct glycoprotein(the optimization saturated degree of saturated ammonium sulfate is 70%), followed by DEAE Cellulose52 ion exchange chromatography initial purification, then Sephadex G100 further purification.To collect the overlap peak of protein and sugar. It’s the Rana  c. Changbaishanensis oviduct glycoprotein sterling(OGP-Ⅰ) after lyophillization. The result of SDSPAG electrophoresis indicates OGP-Ⅰ is only a colored zone. The molecular weight of glycoprotein was 116 000. The result of SDSPAG electrophoresis combined with coomassie brilliant blue staining and shiff staining provs it is glycoprotein, OGP-Ⅰ is only a colored zone at the same local. The ultraviolet scanning of OGP-Ⅰ indicates that it exhibits polysaccharide characteristic absorption band and protein absorption band. Therefore
, these can further indicate that OGP-Ⅰ is glycoprotein.

Key words: Rana chensinensis Changbaishanensis, oviduct glycoprotein, separation, purification, identify