Abstract:

An HPLC method was used to determine the mass ratioes of songoramine and songorine in the fibrous roots of Aconitum carmichaeli Debx. A Zorbax ExtendC18(4.6 mm×250 mm ODS, 5 μm) column was used. The mobile phase consisted of V(acetonitrile)∶V(w(triethylamine)=01%)=
6∶4 was used for isocratic elution. Flow rate was set at 1.0 mL/min, the wavelength of VWD detector was set at 220 nm and the column temperature was maintained at 25.0 ℃. The linear range of two alkaloids was the same as 0.1—10.0 μg (r₂=0.999 8), the mean recoveries were 998% (RSD=149%, n=6) and 993% (RSD=146%, n=6) respectively. The mass ratioes of songoramine and songorine in the fibrous roots of Aconitum carmichaeli Debx were 57.0,110.0 μg/g respectively.

Key words: fibrous roots of Aconitum carmichaeli Debx, songoramine, songorine, HPLC, mass ratio