Abstract: We obtained the lyophilized products of water extracts by removing oil, water extract, freeze drying pine pollen. The water extracts was diluted with Escherichia coli OP50 to a final mass concentration of 0.5,1.0,2.0 mg/mL to culture Caenorhabditis elegans. By measuring the lifespan, oviposition, swallowing, locomotion of C.elegans, we determined that water extracts of pine pollen had no toxic effects on C.elegans. Through oxidative
stress induced by 10 mmol/L hydrogen peroxide, we determined that 0.5,1.0,2.0 mg/mL water extracts of pine pollen had a significant protective effect on oxidative stress of C.elegans.

Key words: antiaging, anti\, Caenorhabditis elegans, oxidant,  pine pollen