Abstract: The artificially cultivated meat-like Ganoderma lucidum (MGL) extracts were applied to the oxidative damage model of Caenorhabditis elegans  to evaluate their toxicity and antioxidant efficacy.  The ultraviolet (UV) full wavelength scanning method was used to determine the main components of the extracts, the life span and spawning quantity were used to evaluate the toxicity of the extracts, the free radical content was detected by active oxygen detection (DCF) method to evaluate the antioxidant efficacy of the extracts, the potential acting target of the extracts was explored by the transcriptome analysis. The experimental results show that the main components of the MGL extracts are quinones and proteins, the extracts have no obvious toxic effects, 2.5 mg/mL extracts have significant antioxidant capacity against damaged nematodes and can effectively regulate the expression of  related genes to the heat shock protein family (hsp-16.1,16.11,16.48,16.49), superoxide dismutase (sod-5), inner mitochondrial membrane (timm-23), metal sulphurin-1 (mtl-1), and the Skp1 protein family.The artificially cultivated MGL extracts can exert obvious antioxidant efficacy through the life\|regulating signal pathway, and  have the potential ability to regulate fat metabolism and promote protein synthesis.

Key words: meat-like Ganoderma lucidum, extract, Caenorhabditis elegans, toxicity, antioxidation, transcriptomics