超声功率对氧化石墨烯分散性和氧化石墨烯/锌膜复合涂层材料生物性能的影响

doi:10.13481/j.1671-587X.20260529

吉林大学学报(医学版) ›› 2026, Vol. 52 ›› Issue (5): 1474-1482.doi: 10.13481/j.1671-587X.20260529

• 硬 • 上一篇

超声功率对氧化石墨烯分散性和氧化石墨烯/锌膜复合涂层材料生物性能的影响

魏文辉¹,李森楠¹,张琪¹,姜坤¹,马婷²,李朕³(),李琦¹()

^1.石河子大学第一附属医院口腔修复科，新疆石河子 832000
^2.石河子大学第一附属医院口腔内科，新疆石河子 832000
^3.石河子大学第一附属医院中医科，新疆石河子 832000

收稿日期:2025-12-01 接受日期:2026-02-07 出版日期:2026-09-28 发布日期:2026-10-15
通讯作者: 李朕,李琦 E-mail:lzh1203@shzu.edu.cn;liqi236@hsc.pku.edu.cn
作者简介:魏文辉（2000-），男，山西省太原市人，在读硕士研究生，主要从事生物材料基础和应用方面的研究。
基金资助:
新疆生产建设兵团科技局指导性科技项目(2024ZD052);新疆生产建设兵团科技局指导性科技项目(2024ZD067);新疆生产建设兵团科技局指导性科技项目(2023ZD062);石河子大学创新发展专项(CXFZ202309)

Effect of ultrasonic power on dispersibility of graphene oxide and biological properties of graphene oxide/zinc film composite coating material

Wenhui WEI¹,Sennan LI¹,Qi ZHANG¹,Kun JIANG¹,Ting MA²,Zhen LI³(),Qi LI¹()

^1.Department of Prosthodontics，First Affiliated Hospital，Shihezi University，Shihezi 832000，China
^2.Department of Oral Internal Medicine，First Affiliated Hospital，Shihezi University，Shihezi 832000，China
^3.Department of Traditional Chinese Medicine，First Affiliated Hospital，Shihezi University，Shihezi 832000，China

Received:2025-12-01 Accepted:2026-02-07 Online:2026-09-28 Published:2026-10-15
Contact: Zhen LI,Qi LI E-mail:lzh1203@shzu.edu.cn;liqi236@hsc.pku.edu.cn

摘要/Abstract

摘要：

目的探讨超声功率对氧化石墨烯（GO）分散性的调控作用，揭示分散性与GO/锌膜复合涂层材料微观结构及生物性能的关联，优化GO基生物医用涂层制备工艺。方法以纯度≥99%的GO粉末和锌膜为原料，采用搅拌同步超声分散-浸渍涂覆工艺制备GO分散体系和GO/锌膜复合涂层材料，设置分为100、300和600 W 3个超声功率梯度。采用扫描电子显微镜（SEM）和拉曼光谱仪分析不同超声功率下各组GO/锌膜复合涂层材料的GO表面分散形态、表面片径、涂层孔隙率及结构缺陷，活/死细胞染色法和细胞计数试剂盒8（CCK-8）法评估不同超声功率处理后GO分散液作用下各组小鼠间充质干细胞（MSCs）存活情况及增殖活性，平板计数法测定各组GO分散液处理后大肠杆菌（E.coli）和金黄色葡萄球菌（S.aureus）存活率。结果 SEM观察，超声分散后的GO涂层可在锌膜表面实现均匀、连续的覆盖，其中300 W组GO/锌膜涂层材料表面平整致密。300 W组GO/锌膜涂层材料表面片径和孔隙率适中，二者均低于100 W组且高于600 W组（P<0.05）。拉曼光谱仪，300 W组GO/锌膜涂层材料拉曼光谱中缺陷峰与石墨峰强度比（I_D/I_G）比值高于100 W组，低于600 W组；其石墨化程度和结构缺陷处于适中水平。活/死细胞染色，300 W组MSCs中存活细胞占比高，细胞铺展良好且伪足清晰；100 W组MSCs中存在少量死亡细胞，存活细胞胞体收缩、伸展范围窄，600 W组MSCs中死亡细胞数较100 W组和300 W组增加。CCK-8法，干预108 h后，与100 W组和600 W组比较，300 W组小鼠MSCs增殖活性明显升高（P<0.05）。平板计数法，与100 W组比较，300 W组E.coli和S.aureus存活率均明显降低（P<0.05）；与600 W组比较，300 W组S.aureus存活率明显降低（P<0.05）。结论 300 W为制备GO/锌膜复合涂层材料的最优超声功率，该参数可通过精准调控GO分散性，同步优化涂层界面结合性能和生物活性，实现生物安全性与抗菌性能的协同提升，为GO基生物医用涂层的工业化生产及临床转化提供关键工艺参数与理论依据。

关键词: 氧化石墨烯, 超声功率, 分散性, 生物性能, 抗菌性能

Abstract:

Objective To explore the regulatory effect of ultrasonic power on the dispersibility of graphene oxide （GO）， to reveal the correlation between GO dispersibility and the microstructure as well as biological properties of GO/zinc film composite coating materials， and to optimize the preparation process of GO-based biomedical coatings. Methods GO powder （purity≥99%） and zinc film were used as raw materials. GO dispersion systems and GO/zinc film composite coating materials were prepared by a stirring-coupled ultrasonic dispersion-dip coating process， with three ultrasonic power gradients set at 100 W， 300 W， and 600 W. Scanning electron microscope （SEM） and Raman spectroscopy were used to analyze the surface dispersion morphology， surface sheet size， coating porosity， and structural defects of GO on the GO/zinc film composite coating materials under different ultrasonic powers. Live/dead cell staining and cell counting kit-8 （CCK-8） method were used to evaluate the survival and the proliferative activities of mouse mesenchymal stem cells （MSCs） in various groups treated with GO dispersions prepared under different ultrasonic powers. Plate counting method was used to determine the survival rates of Escherichia coli （E.coli） and Staphylococcus aureus （S.aureus） in various groups after treatment with GO dispersions prepared under different ultrasonic powers. Results The SEM observation showed that the ultrasonically dispersed GO coating achieved uniform and continuous coverage on zinc film surface； the GO/zinc film coating material in 300 W group exhibited a smooth and dense surface. The surface sheet size and rate of porosity of the GO/zinc film material in 300 W group were moderate， which were lower than those in 100 W group and higher than those in 600 W group （P<0.05）. The Raman spectroscopy analysis results revealed that the ratio of intensities of defect peak and graphitic peak （I_D/I_G） of the GO/zinc film material in 300 W group was higher than that in 100 W group but lower than that in 600 W group， indicating moderate graphitization degree and structural defects. The results of live/dead cell staining showed that a high proportion of survived cells with well-spread morphology and clear pseudopodia was observed in MSCs in 300 W group； a small number of dead cells， with surviving cells exhibiting contracted cell bodies and limited spreading range was observed in the MSCs in 100 W group， while more dead cells were seen in the MSCs in 600 W group. The results of CCK-8 method demonstrated that after 108 h of intervention， compared with 100 W group and 600 W group， the proliferative activity of mouse MSCs in 300 W group was significantly increased （P<0.05）. The results of plate counting method showed that compared with 100 W group， the survival rates of E.coli and S.aureus in 300 W group were significantly decreased （P<0.05）； compared with 600 W group， the survival rate of S.aureus in 300 W group was significantly decreased （P<0.05）. Conclusion 300 W is identified as the optimal ultrasonic power for preparing GO/zinc film composite coating materials. This parameter can precisely regulate the dispersibility of GO， simultaneously optimize the interfacial bonding performance and biological activity of the coating， and synergistically improve biosafety and antibacterial performance. The findings provide the key process parameters and theoretical basis for the industrial production and clinical translation of GO-based biomedical coatings.

Key words: Graphene oxide, Ultrasonic power, Dispersibility, Biological property, Antibacterial performance

中图分类号:

R318.08

魏文辉,李森楠,张琪,姜坤,马婷,李朕,李琦. 超声功率对氧化石墨烯分散性和氧化石墨烯/锌膜复合涂层材料生物性能的影响[J]. 吉林大学学报(医学版), 2026, 52(5): 1474-1482.

Wenhui WEI,Sennan LI,Qi ZHANG,Kun JIANG,Ting MA,Zhen LI,Qi LI. Effect of ultrasonic power on dispersibility of graphene oxide and biological properties of graphene oxide/zinc film composite coating material[J]. Journal of Jilin University(Medicine Edition), 2026, 52(5): 1474-1482.

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Group	Surface sheet diameter（l/μm）	Rate of porosity（η/%）
100 W	0.756±0.166	7.430±0.901
300 W	0.463±0.125^*	2.542±0.310^*
600 W	0.141±0.035^*△	0.857±0.104^*△

Group	Proliferation activity
Group	（t/h） 24	48	108
Control	100.00±0.00	100.00±0.00	100.00±0.00
100 W	104.60±4.93	115.74±2.97^*	118.87±6.62^*
300 W	120.74±5.15^*△	137.41±4.76^*△	129.45±1.91^*△
600 W	96.51±5.05^△#	82.45±2.91^*△#	85.74±4.35^*△#

Group	Survival rate
Group	E.coli	S.aureus
Control	100.00±0.00	100.00±0.00
100 W	62.23±2.61^*	52.24±2.72^*
300 W	34.63±4.23^*△	24.15±0.22^*△
600 W	40.03±1.45^*△	37.32±4.14^*△#

超声功率对氧化石墨烯分散性和氧化石墨烯/锌膜复合涂层材料生物性能的影响

Effect of ultrasonic power on dispersibility of graphene oxide and biological properties of graphene oxide/zinc film composite coating material

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